7eop: Difference between revisions
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==Crystal structure of the Pepper aptamer in complex with HBC620== | |||
<StructureSection load='7eop' size='340' side='right'caption='[[7eop]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7eop]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EOP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=J93:4-[(~{Z})-1-cyano-2-[5-[2-hydroxyethyl(methyl)amino]thieno[3,2-b]thiophen-2-yl]ethenyl]benzenecarbonitrile'>J93</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eop OCA], [https://pdbe.org/7eop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eop RCSB], [https://www.ebi.ac.uk/pdbsum/7eop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eop ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pepper fluorescent RNAs are a recently reported bright, stable and multicolor fluorogenic aptamer tag that enable imaging of diverse RNAs in live cells. To investigate the molecular basis of the superior properties of Pepper, we determined the structures of complexes of Pepper aptamer bound with its cognate HBC or HBC-like fluorophores at high resolution by X-ray crystallography. The Pepper aptamer folds in a monomeric non-G-quadruplex tuning-fork-like architecture composed of a helix and one protruded junction region. The near-planar fluorophore molecule intercalates in the middle of the structure and is sandwiched between one non-G-quadruplex base quadruple and one noncanonical G.U wobble helical base pair. In addition, structure-based mutational analysis is evaluated by in vitro and live-cell fluorogenic detection. Taken together, our research provides a structural basis for demystifying the fluorescence activation mechanism of Pepper aptamer and for further improvement of its future application in RNA visualization. | |||
Structure-based investigation of fluorogenic Pepper aptamer.,Huang K, Chen X, Li C, Song Q, Li H, Zhu L, Yang Y, Ren A Nat Chem Biol. 2021 Dec;17(12):1289-1295. doi: 10.1038/s41589-021-00884-6. Epub, 2021 Nov 1. PMID:34725509<ref>PMID:34725509</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Huang | <div class="pdbe-citations 7eop" style="background-color:#fffaf0;"></div> | ||
[[Category: Ren | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Huang KY]] | |||
[[Category: Ren AM]] | |||
Latest revision as of 19:58, 29 November 2023
Crystal structure of the Pepper aptamer in complex with HBC620Crystal structure of the Pepper aptamer in complex with HBC620
Structural highlights
Publication Abstract from PubMedPepper fluorescent RNAs are a recently reported bright, stable and multicolor fluorogenic aptamer tag that enable imaging of diverse RNAs in live cells. To investigate the molecular basis of the superior properties of Pepper, we determined the structures of complexes of Pepper aptamer bound with its cognate HBC or HBC-like fluorophores at high resolution by X-ray crystallography. The Pepper aptamer folds in a monomeric non-G-quadruplex tuning-fork-like architecture composed of a helix and one protruded junction region. The near-planar fluorophore molecule intercalates in the middle of the structure and is sandwiched between one non-G-quadruplex base quadruple and one noncanonical G.U wobble helical base pair. In addition, structure-based mutational analysis is evaluated by in vitro and live-cell fluorogenic detection. Taken together, our research provides a structural basis for demystifying the fluorescence activation mechanism of Pepper aptamer and for further improvement of its future application in RNA visualization. Structure-based investigation of fluorogenic Pepper aptamer.,Huang K, Chen X, Li C, Song Q, Li H, Zhu L, Yang Y, Ren A Nat Chem Biol. 2021 Dec;17(12):1289-1295. doi: 10.1038/s41589-021-00884-6. Epub, 2021 Nov 1. PMID:34725509[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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