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==The crystal structure of Escherichia coli MurR in complex with N-acetylglucosamine-6-phosphate== | ==The crystal structure of Escherichia coli MurR in complex with N-acetylglucosamine-6-phosphate== | ||
<StructureSection load='7en5' size='340' side='right'caption='[[7en5]]' scene=''> | <StructureSection load='7en5' size='340' side='right'caption='[[7en5]], [[Resolution|resolution]] 1.25Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EN5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EN5 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7en5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EN5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EN5 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7en5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7en5 OCA], [https://pdbe.org/7en5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7en5 RCSB], [https://www.ebi.ac.uk/pdbsum/7en5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7en5 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4QY:BETA-N-ACETYLGLUCOSAMINE-6-PHOSPHATE'>4QY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MXE:2-METHOXYETHANOL'>MXE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7en5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7en5 OCA], [https://pdbe.org/7en5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7en5 RCSB], [https://www.ebi.ac.uk/pdbsum/7en5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7en5 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/MURR_ECOLI MURR_ECOLI] Represses the expression of the murPQ operon involved in the uptake and degradation of N-acetylmuramic acid (MurNAc). Binds to two adjacent inverted repeats within the operator region. MurNAc 6-phosphate, the substrate of MurQ, is the specific inducer that weakens binding of MurR to the operator. Also represses its own transcription.<ref>PMID:18723630</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cell-wall recycling process is important for bacterial survival in nutrient-limited conditions and, in certain cases, is directly involved in antibiotic resistance. In the sophisticated cell-wall recycling process in Escherichia coli, the transcriptional repressor MurR controls the expression of murP and murQ, which are involved in transporting and metabolizing N-acetylmuramic acid (MurNAc), generating N-acetylmuramic acid-6-phosphate (MurNAc-6-P) and N-acetylglucosamine-6-phosphate (GlcNAc-6-P). Here, we report that both MurNAc-6-P and GlcNAc-6-P can bind to MurR and weaken the DNA binding ability of MurR. Structural characterizations of MurR in complex with MurNAc-6-P or GlcNAc-6-P as well as in the apo form revealed the detailed ligand recognition chemistries. Further studies showed that only MurNAc-6-P, but not GlcNAc-6-P, is capable of derepressing the expression of murQP controlled by MurR in cells and clarified the substrate specificity through the identification of key residues responsible for ligand binding in the complex structures. In summary, this study deciphered the molecular mechanism of the cell wall recycling process regulated by MurR in E. coli. | |||
Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli.,Zhang Y, Chen W, Wu D, Liu Y, Wu Z, Li J, Zhang SY, Ji Q Nucleic Acids Res. 2022 Jun 10;50(10):5948-5960. doi: 10.1093/nar/gkac442. PMID:35640608<ref>PMID:35640608</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7en5" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Escherichia coli K-12]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chen W]] | [[Category: Chen W]] | ||
[[Category: Ji Q]] | [[Category: Ji Q]] | ||
[[Category: Zhang Y]] | [[Category: Zhang Y]] | ||
Latest revision as of 19:57, 29 November 2023
The crystal structure of Escherichia coli MurR in complex with N-acetylglucosamine-6-phosphateThe crystal structure of Escherichia coli MurR in complex with N-acetylglucosamine-6-phosphate
Structural highlights
FunctionMURR_ECOLI Represses the expression of the murPQ operon involved in the uptake and degradation of N-acetylmuramic acid (MurNAc). Binds to two adjacent inverted repeats within the operator region. MurNAc 6-phosphate, the substrate of MurQ, is the specific inducer that weakens binding of MurR to the operator. Also represses its own transcription.[1] Publication Abstract from PubMedThe cell-wall recycling process is important for bacterial survival in nutrient-limited conditions and, in certain cases, is directly involved in antibiotic resistance. In the sophisticated cell-wall recycling process in Escherichia coli, the transcriptional repressor MurR controls the expression of murP and murQ, which are involved in transporting and metabolizing N-acetylmuramic acid (MurNAc), generating N-acetylmuramic acid-6-phosphate (MurNAc-6-P) and N-acetylglucosamine-6-phosphate (GlcNAc-6-P). Here, we report that both MurNAc-6-P and GlcNAc-6-P can bind to MurR and weaken the DNA binding ability of MurR. Structural characterizations of MurR in complex with MurNAc-6-P or GlcNAc-6-P as well as in the apo form revealed the detailed ligand recognition chemistries. Further studies showed that only MurNAc-6-P, but not GlcNAc-6-P, is capable of derepressing the expression of murQP controlled by MurR in cells and clarified the substrate specificity through the identification of key residues responsible for ligand binding in the complex structures. In summary, this study deciphered the molecular mechanism of the cell wall recycling process regulated by MurR in E. coli. Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli.,Zhang Y, Chen W, Wu D, Liu Y, Wu Z, Li J, Zhang SY, Ji Q Nucleic Acids Res. 2022 Jun 10;50(10):5948-5960. doi: 10.1093/nar/gkac442. PMID:35640608[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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