7el2: Difference between revisions
No edit summary |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Crystal structure of apo-HpaR from Acinetobacter baumannii== | ==Crystal structure of apo-HpaR from Acinetobacter baumannii== | ||
<StructureSection load='7el2' size='340' side='right'caption='[[7el2]]' scene=''> | <StructureSection load='7el2' size='340' side='right'caption='[[7el2]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EL2 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7el2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EL2 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7el2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7el2 OCA], [https://pdbe.org/7el2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7el2 RCSB], [https://www.ebi.ac.uk/pdbsum/7el2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7el2 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7el2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7el2 OCA], [https://pdbe.org/7el2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7el2 RCSB], [https://www.ebi.ac.uk/pdbsum/7el2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7el2 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A4Q4GPX4_ACIBA A0A4Q4GPX4_ACIBA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
HpaR is a transcription regulator in the MarR family that controls the expression of the gene cluster responsible for conversion of p-hydroxyphenylacetate to pyruvate and succinate for cellular metabolism. Here, we report the biochemical and structural characterization of Acinetobacter baumannii HpaR (AbHpaR) and its complex with cognate DNA. Our study revealed that AbHpaR binds upstream of the divergently transcribed hpaA gene and the meta-cleavage operon, as well as the hpaR gene, thereby repressing their transcription by blocking access of RNA polymerase. Structural analysis of AbHpaR-DNA complex revealed that the DNA binding specificity can be achieved via a combination of both direct and indirect DNA sequence readouts. DNA binding of AbHpaR is weakened by 3,4-dihydroxyphenylacetate (DHPA), which is the substrate of the meta-cleavage reactions; this likely leads to expression of the target genes. Based on our findings, we propose a model for how A. baumannii controls transcription of HPA-metabolizing genes, which highlights the independence of global catabolite repression and could be beneficial for metabolic engineering towards bioremediation applications. | |||
Mechanism of transcription regulation by Acinetobacter baumannii HpaR in the catabolism of p-hydroxyphenylacetate.,Permsirivisarn P, Yuenyao A, Pramanpol N, Charoenwattanasatien R, Suginta W, Chaiyen P, Pakotiprapha D FEBS J. 2021 Dec 30. doi: 10.1111/febs.16340. PMID:34967505<ref>PMID:34967505</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7el2" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Acinetobacter baumannii]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Pakotiprapha D]] | [[Category: Pakotiprapha D]] | ||
[[Category: Permsirivisarn P]] | [[Category: Permsirivisarn P]] | ||