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==== | ==crystal structure of maize SHH2 SAWADEE domain in complex with and H3K9me1 peptide== | ||
<StructureSection load='7ef1' size='340' side='right'caption='[[7ef1]]' scene=''> | <StructureSection load='7ef1' size='340' side='right'caption='[[7ef1]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ef1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EF1 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ef1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ef1 OCA], [https://pdbe.org/7ef1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ef1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ef1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ef1 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ef1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ef1 OCA], [https://pdbe.org/7ef1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ef1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ef1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ef1 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/B7ZYP9_MAIZE B7ZYP9_MAIZE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
RNA-directed DNA methylation (RdDM) is a plant-specific de novo DNA methylation pathway, which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2 (SHH2) is an H3K9me1 reader. Our structural studies reveal that H3K9me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di- and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a mono-methyllysine recognition mechanism which potentially links RdDM to H3K9me1 in maize. | |||
Recognition of H3K9me1 by maize RNA-directed DNA methylation factor SHH2.,Wang Y, Zhou X, Luo J, Lv S, Liu R, Du X, Jia B, Yuan F, Zhang H, Du J J Integr Plant Biol. 2021 Jun;63(6):1091-1096. doi: 10.1111/jipb.13103. Epub 2021, Jun 3. PMID:33913587<ref>PMID:33913587</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ef1" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Zea mays]] | ||
[[Category: Du J]] | |||
[[Category: Wang Y]] | |||
Latest revision as of 19:51, 29 November 2023
crystal structure of maize SHH2 SAWADEE domain in complex with and H3K9me1 peptidecrystal structure of maize SHH2 SAWADEE domain in complex with and H3K9me1 peptide
Structural highlights
FunctionPublication Abstract from PubMedRNA-directed DNA methylation (RdDM) is a plant-specific de novo DNA methylation pathway, which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2 (SHH2) is an H3K9me1 reader. Our structural studies reveal that H3K9me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di- and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a mono-methyllysine recognition mechanism which potentially links RdDM to H3K9me1 in maize. Recognition of H3K9me1 by maize RNA-directed DNA methylation factor SHH2.,Wang Y, Zhou X, Luo J, Lv S, Liu R, Du X, Jia B, Yuan F, Zhang H, Du J J Integr Plant Biol. 2021 Jun;63(6):1091-1096. doi: 10.1111/jipb.13103. Epub 2021, Jun 3. PMID:33913587[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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