7ear: Difference between revisions
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==A positively charged mutant Cry3Aa endotoxin== | |||
<StructureSection load='7ear' size='340' side='right'caption='[[7ear]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7ear]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EAR FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ear FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ear OCA], [https://pdbe.org/7ear PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ear RCSB], [https://www.ebi.ac.uk/pdbsum/7ear PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ear ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9S6N9_BACTU Q9S6N9_BACTU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Direct delivery of proteins into cells holds significant potential for basic research and drug development. However, the poor endosomal escape of conventional delivery strategies remains a challenge, thus limiting the clinical translation of many protein therapeutics. Herein, we report that engineered Cry3Aa protein (Pos3Aa) crystals formed naturally within Bacillus thuringiensis can serve as a vehicle for efficient cytosolic delivery of bioactive proteins. We showed that Pos3Aa-mediated delivery of tumor suppressor p53 protein, a promising therapeutic candidate found to be inactivated in nearly half of human cancers, resulted in the restoration of p53 function in p53-deficient cancer cells, and thereby sensitized them to 5-fluorouracil chemotherapy as demonstrated in in vitro and in vivo models. Our results validate that Pos3Aa crystals can be a robust and effective platform for the cytosolic delivery of effector proteins, and suggest that efficient uptake and endosomal escape could be critical for efficacious p53 protein-based cancer therapy. | |||
Efficient intracellular delivery of p53 protein by engineered protein crystals restores tumor suppressing function in vivo.,Yang Z, Lee MMM, Chan MK Biomaterials. 2021 Apr;271:120759. doi: 10.1016/j.biomaterials.2021.120759. Epub , 2021 Mar 16. PMID:33798968<ref>PMID:33798968</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7ear" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Pesticidal crystal protein|Pesticidal crystal protein]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bacillus thuringiensis]] | |||
[[Category: Large Structures]] | |||
[[Category: Chan MK]] | |||
[[Category: Lee MM]] | |||
[[Category: Yang Z]] | |||
Latest revision as of 19:49, 29 November 2023
A positively charged mutant Cry3Aa endotoxinA positively charged mutant Cry3Aa endotoxin
Structural highlights
FunctionPublication Abstract from PubMedDirect delivery of proteins into cells holds significant potential for basic research and drug development. However, the poor endosomal escape of conventional delivery strategies remains a challenge, thus limiting the clinical translation of many protein therapeutics. Herein, we report that engineered Cry3Aa protein (Pos3Aa) crystals formed naturally within Bacillus thuringiensis can serve as a vehicle for efficient cytosolic delivery of bioactive proteins. We showed that Pos3Aa-mediated delivery of tumor suppressor p53 protein, a promising therapeutic candidate found to be inactivated in nearly half of human cancers, resulted in the restoration of p53 function in p53-deficient cancer cells, and thereby sensitized them to 5-fluorouracil chemotherapy as demonstrated in in vitro and in vivo models. Our results validate that Pos3Aa crystals can be a robust and effective platform for the cytosolic delivery of effector proteins, and suggest that efficient uptake and endosomal escape could be critical for efficacious p53 protein-based cancer therapy. Efficient intracellular delivery of p53 protein by engineered protein crystals restores tumor suppressing function in vivo.,Yang Z, Lee MMM, Chan MK Biomaterials. 2021 Apr;271:120759. doi: 10.1016/j.biomaterials.2021.120759. Epub , 2021 Mar 16. PMID:33798968[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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