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==Crystal structure of Trypanosoma brucei cathepsin B Y217C/S275C mutant== | ==Crystal structure of Trypanosoma brucei cathepsin B Y217C/S275C mutant== | ||
<StructureSection load='7e3f' size='340' side='right'caption='[[7e3f]]' scene=''> | <StructureSection load='7e3f' size='340' side='right'caption='[[7e3f]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E3F FirstGlance]. <br> | <table><tr><td colspan='2'>[[7e3f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei_TREU927 Trypanosoma brucei brucei TREU927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E3F FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e3f OCA], [https://pdbe.org/7e3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e3f RCSB], [https://www.ebi.ac.uk/pdbsum/7e3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e3f ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e3f OCA], [https://pdbe.org/7e3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e3f RCSB], [https://www.ebi.ac.uk/pdbsum/7e3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e3f ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/D6XHE1_TRYB2 D6XHE1_TRYB2] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Protein assemblies can be designed for development of nano-bio materials. This has been achieved by modulating protein-protein interactions. However, fabrication of highly ordered protein assemblies remains challenging. Protein crystals, which have highly ordered arrangements of protein molecules, provide useful source matrices for synthesizing artificial protein assemblies. Here, we describe construction of a supramolecular filament structure by engineering covalent and non-covalent interactions in a protein crystal. Performing in-cell crystallization of Trypanosoma brucei cysteine protease cathepsin B (TbCatB), we achieved a precise arrangement of protein molecules while suppressing random aggregation due to disulfide bonds. We succeeded in synthesizing bundled filament from the crystals by autoxidation of cysteinyl thiols after the isolation of the crystals from living cells. | |||
Design of an In-Cell Protein Crystal for the Environmentally Responsive Construction of a Supramolecular Filament.,Abe S, Pham TT, Negishi H, Yamashita K, Hirata K, Ueno T Angew Chem Int Ed Engl. 2021 May 25;60(22):12341-12345. doi:, 10.1002/anie.202102039. Epub 2021 Apr 23. PMID:33759310<ref>PMID:33759310</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7e3f" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Cathepsin 3D structures|Cathepsin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Trypanosoma brucei brucei TREU927]] | |||
[[Category: Abe S]] | [[Category: Abe S]] | ||
[[Category: Hirata K]] | [[Category: Hirata K]] | ||
Latest revision as of 19:45, 29 November 2023
Crystal structure of Trypanosoma brucei cathepsin B Y217C/S275C mutantCrystal structure of Trypanosoma brucei cathepsin B Y217C/S275C mutant
Structural highlights
FunctionPublication Abstract from PubMedProtein assemblies can be designed for development of nano-bio materials. This has been achieved by modulating protein-protein interactions. However, fabrication of highly ordered protein assemblies remains challenging. Protein crystals, which have highly ordered arrangements of protein molecules, provide useful source matrices for synthesizing artificial protein assemblies. Here, we describe construction of a supramolecular filament structure by engineering covalent and non-covalent interactions in a protein crystal. Performing in-cell crystallization of Trypanosoma brucei cysteine protease cathepsin B (TbCatB), we achieved a precise arrangement of protein molecules while suppressing random aggregation due to disulfide bonds. We succeeded in synthesizing bundled filament from the crystals by autoxidation of cysteinyl thiols after the isolation of the crystals from living cells. Design of an In-Cell Protein Crystal for the Environmentally Responsive Construction of a Supramolecular Filament.,Abe S, Pham TT, Negishi H, Yamashita K, Hirata K, Ueno T Angew Chem Int Ed Engl. 2021 May 25;60(22):12341-12345. doi:, 10.1002/anie.202102039. Epub 2021 Apr 23. PMID:33759310[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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