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==Crystal structure of multidrug efflux transporter OqxB from Klebsiella pneumoniae== | ==Crystal structure of multidrug efflux transporter OqxB from Klebsiella pneumoniae== | ||
<StructureSection load='7cz9' size='340' side='right'caption='[[7cz9]]' scene=''> | <StructureSection load='7cz9' size='340' side='right'caption='[[7cz9]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CZ9 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7cz9]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CZ9 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cz9 OCA], [https://pdbe.org/7cz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cz9 RCSB], [https://www.ebi.ac.uk/pdbsum/7cz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cz9 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=PTY:PHOSPHATIDYLETHANOLAMINE'>PTY</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cz9 OCA], [https://pdbe.org/7cz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cz9 RCSB], [https://www.ebi.ac.uk/pdbsum/7cz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cz9 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/U5U6L7_KLEPN U5U6L7_KLEPN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
OqxB is an RND (Resistance-Nodulation-Division) efflux pump that has emerged as a factor contributing to the antibiotic resistance in Klebsiella pneumoniae. OqxB underwent horizontal gene transfer and is now seen in other Gram-negative bacterial pathogens including Escherichia coli, Enterobacter cloacae and Salmonella spp., further disseminating multi-drug resistance. In this study, we describe crystal structure of OqxB with n-dodecyl-beta-D-maltoside (DDM) molecules bound in its substrate-binding pocket, at 1.85 A resolution. We utilize this structure in computational studies to predict the key amino acids contributing to the efflux of fluoroquinolones by OqxB, distinct from analogous residues in related transporters AcrB and MexB. Finally, our complementation assays with mutated OqxB and minimum inhibitory concentration (MIC) experiments with clinical isolates of E. coli provide further evidence that the predicted structural features are indeed involved in ciprofloxacin efflux. | |||
Structure and function relationship of OqxB efflux pump from Klebsiella pneumoniae.,Bharatham N, Bhowmik P, Aoki M, Okada U, Sharma S, Yamashita E, Shanbhag AP, Rajagopal S, Thomas T, Sarma M, Narjari R, Nagaraj S, Ramachandran V, Katagihallimath N, Datta S, Murakami S Nat Commun. 2021 Sep 13;12(1):5400. doi: 10.1038/s41467-021-25679-0. PMID:34518546<ref>PMID:34518546</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7cz9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Klebsiella pneumoniae]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Murakami S]] | [[Category: Murakami S]] | ||
[[Category: Okada U]] | [[Category: Okada U]] | ||
[[Category: Yamashita E]] | [[Category: Yamashita E]] | ||