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==Structural and functional analysis of small heat shock protein from Synechococcus phage S-ShM2== | ==Structural and functional analysis of small heat shock protein from Synechococcus phage S-ShM2== | ||
<StructureSection load='7ck4' size='340' side='right'caption='[[7ck4]]' scene=''> | <StructureSection load='7ck4' size='340' side='right'caption='[[7ck4]], [[Resolution|resolution]] 7.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CK4 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ck4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_phage_S-ShM2 Synechococcus phage S-ShM2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CK4 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ck4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ck4 OCA], [https://pdbe.org/7ck4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ck4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ck4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ck4 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 7Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ck4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ck4 OCA], [https://pdbe.org/7ck4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ck4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ck4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ck4 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/E3SJI7_9CAUD E3SJI7_9CAUD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structures of a cyanophage small heat shock protein (sHSP) were determined as octahedrons of 24-mers and 48-mers and as icosahedrons of 60-mers. An N-terminal deletion construct of an 18 kDa sHSP of Synechococcus sp. phage S-ShM2 crystallized as a 24-mer and its structure was determined at a resolution of 7 A. The negative stain electron microscopy (EM) images showed that the full-length protein is a mixture of a major population of larger and a minor population of smaller cage-like particles. Their structures have been determined by electron cryomicroscopy 3D image reconstruction at a resolution of 8 A. The larger particles are 60-mers with icosahedral symmetry and the smaller ones are 48-mers with octahedral symmetry. These structures are the first of the viral/phage origin and the 60-mer is the largest and the first icosahedral assembly to be reported for sHSPs. | |||
Multiple nanocages of a cyanophage small heat shock protein with icosahedral and octahedral symmetries.,Biswas S, Garg P, Dutta S, Suguna K Sci Rep. 2021 Oct 25;11(1):21023. doi: 10.1038/s41598-021-00172-2. PMID:34697325<ref>PMID:34697325</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ck4" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Heat Shock Protein structures|Heat Shock Protein structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Synechococcus phage S-ShM2]] | |||
[[Category: Biswas S]] | [[Category: Biswas S]] | ||
[[Category: Suguna K]] | [[Category: Suguna K]] | ||
Latest revision as of 19:09, 29 November 2023
Structural and functional analysis of small heat shock protein from Synechococcus phage S-ShM2Structural and functional analysis of small heat shock protein from Synechococcus phage S-ShM2
Structural highlights
FunctionPublication Abstract from PubMedThe structures of a cyanophage small heat shock protein (sHSP) were determined as octahedrons of 24-mers and 48-mers and as icosahedrons of 60-mers. An N-terminal deletion construct of an 18 kDa sHSP of Synechococcus sp. phage S-ShM2 crystallized as a 24-mer and its structure was determined at a resolution of 7 A. The negative stain electron microscopy (EM) images showed that the full-length protein is a mixture of a major population of larger and a minor population of smaller cage-like particles. Their structures have been determined by electron cryomicroscopy 3D image reconstruction at a resolution of 8 A. The larger particles are 60-mers with icosahedral symmetry and the smaller ones are 48-mers with octahedral symmetry. These structures are the first of the viral/phage origin and the 60-mer is the largest and the first icosahedral assembly to be reported for sHSPs. Multiple nanocages of a cyanophage small heat shock protein with icosahedral and octahedral symmetries.,Biswas S, Garg P, Dutta S, Suguna K Sci Rep. 2021 Oct 25;11(1):21023. doi: 10.1038/s41598-021-00172-2. PMID:34697325[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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