7cj0: Difference between revisions
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==== | ==Crystal structure of DNAJC9 HBD in complex with H3.3-H4 dimer and MCM2 HBD== | ||
<StructureSection load='7cj0' size='340' side='right'caption='[[7cj0]]' scene=''> | <StructureSection load='7cj0' size='340' side='right'caption='[[7cj0]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7cj0]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CJ0 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cj0 OCA], [https://pdbe.org/7cj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cj0 RCSB], [https://www.ebi.ac.uk/pdbsum/7cj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cj0 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cj0 OCA], [https://pdbe.org/7cj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cj0 RCSB], [https://www.ebi.ac.uk/pdbsum/7cj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cj0 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/H33_HUMAN H33_HUMAN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
From biosynthesis to assembly into nucleosomes, histones are handed through a cascade of histone chaperones, which shield histones from non-specific interactions. Whether mechanisms exist to safeguard the histone fold during histone chaperone handover events or to release trapped intermediates is unclear. Using structure-guided and functional proteomics, we identify and characterize a histone chaperone function of DNAJC9, a heat shock co-chaperone that promotes HSP70-mediated catalysis. We elucidate the structure of DNAJC9, in a histone H3-H4 co-chaperone complex with MCM2, revealing how this dual histone and heat shock co-chaperone binds histone substrates. We show that DNAJC9 recruits HSP70-type enzymes via its J domain to fold histone H3-H4 substrates: upstream in the histone supply chain, during replication- and transcription-coupled nucleosome assembly, and to clean up spurious interactions. With its dual functionality, DNAJC9 integrates ATP-resourced protein folding into the histone supply pathway to resolve aberrant intermediates throughout the dynamic lives of histones. | |||
DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network.,Hammond CM, Bao H, Hendriks IA, Carraro M, Garcia-Nieto A, Liu Y, Reveron-Gomez N, Spanos C, Chen L, Rappsilber J, Nielsen ML, Patel DJ, Huang H, Groth A Mol Cell. 2021 Jun 17;81(12):2533-2548.e9. doi: 10.1016/j.molcel.2021.03.041. , Epub 2021 Apr 14. PMID:33857403<ref>PMID:33857403</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7cj0" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[DnaJ homolog 3D structures|DnaJ homolog 3D structures]] | |||
*[[Heat Shock Protein structures|Heat Shock Protein structures]] | |||
*[[Histone 3D structures|Histone 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Bao H]] | ||
[[Category: Huang H]] | |||