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==== | ==Crystal structure of DNA-bound CCT/NF-YB/YC complex (HD1CCT/GHD8/OsNF-YC2)== | ||
<StructureSection load='7c9o' size='340' side='right'caption='[[7c9o]]' scene=''> | <StructureSection load='7c9o' size='340' side='right'caption='[[7c9o]], [[Resolution|resolution]] 2.55Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[7c9o]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa] and [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C9O FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c9o OCA], [https://pdbe.org/7c9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c9o RCSB], [https://www.ebi.ac.uk/pdbsum/7c9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c9o ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/HD1_ORYSJ HD1_ORYSJ] Probable transcription factor involved in the regulation of flower development. Required for the promotion of flowering under short day (SD) conditions and the suppression of flowering under long day (LD) conditions. Regulates positively the floral activator HEADING DATE 3a (HD3A) under SD and negatively under LD conditions.<ref>PMID:12700762</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CCT (CONSTANS, CONSTANS-LIKE and TOC1) domain-containing proteins are a large family unique to plants. They transcriptionally regulate the processes related to photoperiodic flowering, circadian rhythms, vernalization, and other related functions. CO (CONSTANS) and HD1 (HEADING DATE 1), through the CCT domain, coordinate with the NF-YB/YC dimer to specifically target a conserved 'CCACA' motif within the downstream promotor. However, the mechanism underlying the DNA recognition by CCT domain remains unclear. Here we determined the crystal structures of the rice NF-YB/YC dimer and the florigen Hd3a-bound HD1CCT/NF-YB/YC trimer with resolutions of 2.0 A and 2.55 A, respectively. The CCT domain of HD1 displays an elongated structure containing two (alpha)-helices and two loops, tethering the Hd3a to the NF-YB/YC dimer. Helix (alpha)2 and loop 2 are anchored into the minor groove of the 'CCACA' motif, which determines the specific base recognition. Our structures reveal the interaction mechanism among the CCT domain, NF-YB/YC dimer, and the target DNA. These results not only provide new insights into the network between the CCT proteins and NF-Y subunits, but also offer references for improving productivity and global adaptability of crops by manipulating florigen expression. | |||
Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering.,Shen C, Liu H, Guan Z, Junjie Y, Ting Z, Yan W, Wu C, Zhang Q, Yin P, Xing Y Plant Cell. 2020 Aug 25. pii: tpc.20.00067. doi: 10.1105/tpc.20.00067. PMID:32843433<ref>PMID:32843433</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7c9o" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Nuclear transcription factor Y|Nuclear transcription factor Y]] | |||
*[[3D structures of constans|3D structures of constans]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Z | [[Category: Oryza sativa]] | ||
[[Category: Oryza sativa Japonica Group]] | |||
[[Category: Guan Z]] | |||
[[Category: Liu H]] | |||
[[Category: Shen C]] | |||
[[Category: Xing Y]] | |||
[[Category: Yin P]] | |||
Latest revision as of 19:00, 29 November 2023
Crystal structure of DNA-bound CCT/NF-YB/YC complex (HD1CCT/GHD8/OsNF-YC2)Crystal structure of DNA-bound CCT/NF-YB/YC complex (HD1CCT/GHD8/OsNF-YC2)
Structural highlights
FunctionHD1_ORYSJ Probable transcription factor involved in the regulation of flower development. Required for the promotion of flowering under short day (SD) conditions and the suppression of flowering under long day (LD) conditions. Regulates positively the floral activator HEADING DATE 3a (HD3A) under SD and negatively under LD conditions.[1] Publication Abstract from PubMedCCT (CONSTANS, CONSTANS-LIKE and TOC1) domain-containing proteins are a large family unique to plants. They transcriptionally regulate the processes related to photoperiodic flowering, circadian rhythms, vernalization, and other related functions. CO (CONSTANS) and HD1 (HEADING DATE 1), through the CCT domain, coordinate with the NF-YB/YC dimer to specifically target a conserved 'CCACA' motif within the downstream promotor. However, the mechanism underlying the DNA recognition by CCT domain remains unclear. Here we determined the crystal structures of the rice NF-YB/YC dimer and the florigen Hd3a-bound HD1CCT/NF-YB/YC trimer with resolutions of 2.0 A and 2.55 A, respectively. The CCT domain of HD1 displays an elongated structure containing two (alpha)-helices and two loops, tethering the Hd3a to the NF-YB/YC dimer. Helix (alpha)2 and loop 2 are anchored into the minor groove of the 'CCACA' motif, which determines the specific base recognition. Our structures reveal the interaction mechanism among the CCT domain, NF-YB/YC dimer, and the target DNA. These results not only provide new insights into the network between the CCT proteins and NF-Y subunits, but also offer references for improving productivity and global adaptability of crops by manipulating florigen expression. Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering.,Shen C, Liu H, Guan Z, Junjie Y, Ting Z, Yan W, Wu C, Zhang Q, Yin P, Xing Y Plant Cell. 2020 Aug 25. pii: tpc.20.00067. doi: 10.1105/tpc.20.00067. PMID:32843433[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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