6m0x: Difference between revisions
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New page: '''Unreleased structure''' The entry 6m0x is ON HOLD Authors: Zhang, Y., Zhang, H., Xu, X., Wang, Y., Chen, W., Wang, Y., Wu, Z., Tang, N., Wang, Y., Zhao, S., Gan, J., Ji, Q. Descript... |
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The | ==Crystal structure of Streptococcus thermophilus Cas9 in complex with AGGA PAM== | ||
<StructureSection load='6m0x' size='340' side='right'caption='[[6m0x]], [[Resolution|resolution]] 2.56Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6m0x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_thermophilus Streptococcus thermophilus] and [https://en.wikipedia.org/wiki/Streptococcus_thermophilus_LMD-9 Streptococcus thermophilus LMD-9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M0X FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.561Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m0x OCA], [https://pdbe.org/6m0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m0x RCSB], [https://www.ebi.ac.uk/pdbsum/6m0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m0x ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CAS9A_STRTD CAS9A_STRTD] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity). Cuts target DNA when Cas9 and gRNAs are mixed.[HAMAP-Rule:MF_01480]<ref>PMID:24270795</ref> | |||
==See Also== | |||
*[[Endonuclease 3D structures|Endonuclease 3D structures]] | |||
== References == | |||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Streptococcus thermophilus]] | ||
[[Category: | [[Category: Streptococcus thermophilus LMD-9]] | ||
[[Category: | [[Category: Chen W]] | ||
[[Category: | [[Category: Gan J]] | ||
[[Category: | [[Category: Ji Q]] | ||
[[Category: | [[Category: Tang N]] | ||
[[Category: | [[Category: Wang Y]] | ||
[[Category: Wu Z]] | |||
[[Category: Xu X]] | |||
[[Category: Zhang H]] | |||
[[Category: Zhang Y]] | |||
[[Category: Zhao S]] | |||
Latest revision as of 18:09, 29 November 2023
Crystal structure of Streptococcus thermophilus Cas9 in complex with AGGA PAMCrystal structure of Streptococcus thermophilus Cas9 in complex with AGGA PAM
Structural highlights
FunctionCAS9A_STRTD CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity). Cuts target DNA when Cas9 and gRNAs are mixed.[HAMAP-Rule:MF_01480][1] See AlsoReferences
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