6c66: Difference between revisions

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-'''Unreleased structure'''
-The entry 6c66 is ON HOLD  until Paper Publication
+==CRISPR RNA-guided surveillance complex, pre-nicking==
+<SX load='6c66' size='340' side='right' viewer='molstar' caption='[[6c66]], [[Resolution|resolution]] 3.66&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6c66]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca] and [https://en.wikipedia.org/wiki/Thermobifida_fusca_YX Thermobifida fusca YX]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C66 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C66 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.66&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c66 OCA], [https://pdbe.org/6c66 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c66 RCSB], [https://www.ebi.ac.uk/pdbsum/6c66 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c66 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q47PJ0_THEFY Q47PJ0_THEFY]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Type I CRISPR-Cas system features a sequential target-searching and degradation process on double-stranded DNA, by the RNA-guided Cascade complex and the nuclease-helicase fusion enzyme Cas3, respectively. Here we present a 3.7 A resolution cryo-EM structure of the Type I-E Cascade/R-loop/Cas3 complex, poised to initiate DNA degradation. Cas3 distinguishes Cascade conformations and only captures the R-loop forming Cascade, to avoid cleaving partially complementary targets. Its nuclease domain recruits the non-target strand (NTS) DNA at a bulged region for the nicking of single-stranded DNA. An additional 4.7 A resolution cryo-EM structure captures the post-nicking state, in which the severed NTS retracts to the helicase entrance, to be threaded for ATP-dependent processive degradation. These snapshots form the basis for understanding RNA-guided DNA degradation in Type I-E CRISPR-Cas systems.
-Authors:
+Structure basis for RNA-guided DNA degradation by Cascade and Cas3.,Xiao Y, Luo M, Dolan AE, Liao M, Ke A Science. 2018 Jun 7. pii: science.aat0839. doi: 10.1126/science.aat0839. PMID:29880725<ref>PMID:29880725</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6c66" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[CRISPR type I-E (Cascade)|CRISPR type I-E (Cascade)]]
+*[[Helicase 3D structures|Helicase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</SX>
+[[Category: Large Structures]]
+[[Category: Thermobifida fusca]]
+[[Category: Thermobifida fusca YX]]
+[[Category: Ke A]]
+[[Category: Liao M]]
+[[Category: Luo M]]
+[[Category: Xiao Y]]