6c66: Difference between revisions
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New page: '''Unreleased structure''' The entry 6c66 is ON HOLD Authors: Description: Category: Unreleased Structures |
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==CRISPR RNA-guided surveillance complex, pre-nicking== | |||
<SX load='6c66' size='340' side='right' viewer='molstar' caption='[[6c66]], [[Resolution|resolution]] 3.66Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6c66]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca] and [https://en.wikipedia.org/wiki/Thermobifida_fusca_YX Thermobifida fusca YX]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C66 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C66 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.66Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c66 OCA], [https://pdbe.org/6c66 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c66 RCSB], [https://www.ebi.ac.uk/pdbsum/6c66 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c66 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q47PJ0_THEFY Q47PJ0_THEFY] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Type I CRISPR-Cas system features a sequential target-searching and degradation process on double-stranded DNA, by the RNA-guided Cascade complex and the nuclease-helicase fusion enzyme Cas3, respectively. Here we present a 3.7 A resolution cryo-EM structure of the Type I-E Cascade/R-loop/Cas3 complex, poised to initiate DNA degradation. Cas3 distinguishes Cascade conformations and only captures the R-loop forming Cascade, to avoid cleaving partially complementary targets. Its nuclease domain recruits the non-target strand (NTS) DNA at a bulged region for the nicking of single-stranded DNA. An additional 4.7 A resolution cryo-EM structure captures the post-nicking state, in which the severed NTS retracts to the helicase entrance, to be threaded for ATP-dependent processive degradation. These snapshots form the basis for understanding RNA-guided DNA degradation in Type I-E CRISPR-Cas systems. | |||
Structure basis for RNA-guided DNA degradation by Cascade and Cas3.,Xiao Y, Luo M, Dolan AE, Liao M, Ke A Science. 2018 Jun 7. pii: science.aat0839. doi: 10.1126/science.aat0839. PMID:29880725<ref>PMID:29880725</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6c66" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[CRISPR type I-E (Cascade)|CRISPR type I-E (Cascade)]] | |||
*[[Helicase 3D structures|Helicase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Thermobifida fusca]] | |||
[[Category: Thermobifida fusca YX]] | |||
[[Category: Ke A]] | |||
[[Category: Liao M]] | |||
[[Category: Luo M]] | |||
[[Category: Xiao Y]] | |||
Latest revision as of 17:33, 29 November 2023
CRISPR RNA-guided surveillance complex, pre-nickingCRISPR RNA-guided surveillance complex, pre-nicking
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