5o09: Difference between revisions
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New page: '''Unreleased structure''' The entry 5o09 is ON HOLD Authors: Deng, X., Bharat, T.A.M., Lowe, J. Description: BtubABC mini microtubule Category: Unreleased Structures [[Category: L... |
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==BtubABC mini microtubule== | |||
<SX load='5o09' size='340' side='right' viewer='molstar' caption='[[5o09]], [[Resolution|resolution]] 3.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5o09]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Prosthecobacter_dejongeii Prosthecobacter dejongeii] and [https://en.wikipedia.org/wiki/Prosthecobacter_vanneervenii Prosthecobacter vanneervenii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O09 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o09 OCA], [https://pdbe.org/5o09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o09 RCSB], [https://www.ebi.ac.uk/pdbsum/5o09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o09 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8GCC5_9BACT Q8GCC5_9BACT] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Microtubules, the dynamic, yet stiff hollow tubes built from alphabeta-tubulin protein heterodimers, are thought to be present only in eukaryotic cells. Here, we report a 3.6-A helical reconstruction electron cryomicroscopy structure of four-stranded mini microtubules formed by bacterial tubulin-like Prosthecobacter dejongeii BtubAB proteins. Despite their much smaller diameter, mini microtubules share many key structural features with eukaryotic microtubules, such as an M-loop, alternating subunits, and a seam that breaks overall helical symmetry. Using in vitro total internal reflection fluorescence microscopy, we show that bacterial mini microtubules treadmill and display dynamic instability, another hallmark of eukaryotic microtubules. The third protein in the btub gene cluster, BtubC, previously known as "bacterial kinesin light chain," binds along protofilaments every 8 nm, inhibits BtubAB mini microtubule catastrophe, and increases rescue. Our work reveals that some bacteria contain regulated and dynamic cytomotive microtubule systems that were once thought to be only useful in much larger and sophisticated eukaryotic cells. | |||
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability.,Deng X, Fink G, Bharat TAM, He S, Kureisaite-Ciziene D, Lowe J Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):E5950-E5958. doi:, 10.1073/pnas.1705062114. Epub 2017 Jul 3. PMID:28673988<ref>PMID:28673988</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5o09" style="background-color:#fffaf0;"></div> | ||
[[Category: Deng | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Prosthecobacter dejongeii]] | |||
[[Category: Prosthecobacter vanneervenii]] | |||
[[Category: Bharat TAM]] | |||
[[Category: Deng X]] | |||
[[Category: Lowe J]] | |||
Latest revision as of 15:18, 22 November 2023
BtubABC mini microtubuleBtubABC mini microtubule
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