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==AMP RNA APTAMER COMPLEX, NMR, 8 STRUCTURES== | |||
<StructureSection load='1am0' size='340' side='right'caption='[[1am0]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1am0]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ara 1ara]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AM0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AM0 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
--> | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1am0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1am0 OCA], [https://pdbe.org/1am0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1am0 RCSB], [https://www.ebi.ac.uk/pdbsum/1am0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1am0 ProSAT]</span></td></tr> | |||
</table> | |||
'''AMP | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | |||
== | |||
The catalytic properties of RNA and its well known role in gene expression and regulation are the consequence of its unique solution structures. Identification of the structural determinants of ligand recognition by RNA molecules is of fundamental importance for understanding the biological functions of RNA, as well as for the rational design of RNA Sequences with specific catalytic activities. Towards this latter end, Szostak et al. used in vitro selection techniques to isolate RNA sequences ('aptamers') containing a high-affinity binding site for ATP, the universal currency of cellular energy, and then used this motif to engineer ribozymes with polynucleotide kinase activity. Here we present the solution structure, as determined by multidimensional NMR spectroscopy and molecular dynamics calculations, of both uniformly and specifically 13C-, 15N-labelled 40-mer RNA containing the ATP-binding motif complexed with AMP. The aptamer adopts an L-shaped structure with two nearly orthogonal stems, each capped proximally by a G x G mismatch pair, binding the AMP ligand at their junction in a GNRA-like motif. | The catalytic properties of RNA and its well known role in gene expression and regulation are the consequence of its unique solution structures. Identification of the structural determinants of ligand recognition by RNA molecules is of fundamental importance for understanding the biological functions of RNA, as well as for the rational design of RNA Sequences with specific catalytic activities. Towards this latter end, Szostak et al. used in vitro selection techniques to isolate RNA sequences ('aptamers') containing a high-affinity binding site for ATP, the universal currency of cellular energy, and then used this motif to engineer ribozymes with polynucleotide kinase activity. Here we present the solution structure, as determined by multidimensional NMR spectroscopy and molecular dynamics calculations, of both uniformly and specifically 13C-, 15N-labelled 40-mer RNA containing the ATP-binding motif complexed with AMP. The aptamer adopts an L-shaped structure with two nearly orthogonal stems, each capped proximally by a G x G mismatch pair, binding the AMP ligand at their junction in a GNRA-like motif. | ||
Structural basis of RNA folding and recognition in an AMP-RNA aptamer complex.,Jiang F, Kumar RA, Jones RA, Patel DJ Nature. 1996 Jul 11;382(6587):183-6. PMID:8700212<ref>PMID:8700212</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1am0" style="background-color:#fffaf0;"></div> | |||
== References == | |||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Jiang F]] | ||
[[Category: Jones RA]] | |||
[[Category: Kumar RA]] | |||
[[Category: Patel DJ]] | |||
Latest revision as of 14:32, 22 November 2023
AMP RNA APTAMER COMPLEX, NMR, 8 STRUCTURESAMP RNA APTAMER COMPLEX, NMR, 8 STRUCTURES
Structural highlights
Publication Abstract from PubMedThe catalytic properties of RNA and its well known role in gene expression and regulation are the consequence of its unique solution structures. Identification of the structural determinants of ligand recognition by RNA molecules is of fundamental importance for understanding the biological functions of RNA, as well as for the rational design of RNA Sequences with specific catalytic activities. Towards this latter end, Szostak et al. used in vitro selection techniques to isolate RNA sequences ('aptamers') containing a high-affinity binding site for ATP, the universal currency of cellular energy, and then used this motif to engineer ribozymes with polynucleotide kinase activity. Here we present the solution structure, as determined by multidimensional NMR spectroscopy and molecular dynamics calculations, of both uniformly and specifically 13C-, 15N-labelled 40-mer RNA containing the ATP-binding motif complexed with AMP. The aptamer adopts an L-shaped structure with two nearly orthogonal stems, each capped proximally by a G x G mismatch pair, binding the AMP ligand at their junction in a GNRA-like motif. Structural basis of RNA folding and recognition in an AMP-RNA aptamer complex.,Jiang F, Kumar RA, Jones RA, Patel DJ Nature. 1996 Jul 11;382(6587):183-6. PMID:8700212[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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