139d: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==SOLUTION STRUCTURE OF A PARALLEL-STRANDED G-QUADRUPLEX DNA== | ==SOLUTION STRUCTURE OF A PARALLEL-STRANDED G-QUADRUPLEX DNA== | ||
<StructureSection load='139d' size='340' side='right'caption='[[139d | <StructureSection load='139d' size='340' side='right'caption='[[139d]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[139d]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=139D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=139D FirstGlance]. <br> | <table><tr><td colspan='2'>[[139d]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=139D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=139D FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=139d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=139d OCA], [https://pdbe.org/139d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=139d RCSB], [https://www.ebi.ac.uk/pdbsum/139d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=139d ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=139d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=139d OCA], [https://pdbe.org/139d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=139d RCSB], [https://www.ebi.ac.uk/pdbsum/139d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=139d ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
| Line 20: | Line 21: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Patel | [[Category: Patel DJ]] | ||
[[Category: Wang | [[Category: Wang Y]] | ||
Latest revision as of 14:31, 22 November 2023
SOLUTION STRUCTURE OF A PARALLEL-STRANDED G-QUADRUPLEX DNASOLUTION STRUCTURE OF A PARALLEL-STRANDED G-QUADRUPLEX DNA
Structural highlights
Publication Abstract from PubMedThis paper reports on the solution structure of a parallel-stranded G-quadruplex formed by the Tetrahymena telomeric sequence d(T-T-G-G-G-G-T) whose NMR parameters in potassium cation containing solution were previously published from our laboratory. The structure was determined by combining a quantitative analysis of the NMR data with molecular dynamics calculations including relaxation matrix refinement. The combined NMR-computational approach yielded a set of seven distance-refined structures with pairwise RMSDs ranging from 0.66 to 1.30 A for the central G-G-G-G tetranucleotide segment. Four of the seven structures were refined further using complete relaxation-matrix calculations to yield solution structures with pairwise RMSDs ranging from 0.64 to 1.04 A for the same tetranucleotide segment. The R-factors also decreased on proceeding from the distance-refined to relaxation matrix-refined structures. The four strands of the G-quadruplex are aligned in parallel and are related by a 4-fold symmetry axis coincident with the helix axis. Individual guanines from each strand form planar G.G.G.G tetrad arrangements with each tetrad stabilized by eight hydrogen bonds involving the Watson-Crick and Hoogsteen edges of the guanine bases. All guanines adopt anti glycosidic torsion angles and S type sugar puckers in this right-handed parallel-stranded G-quadruplex structure. The four G.G.G.G tetrad planes stack on each other with minimal overlap of adjacent guanine base planes within individual strands. The thymine residues are under-defined in the solution structure of the d(T-T-G-G-G-G-T) G-quadruplex and sample amongst multiple conformations in solution. Solution structure of a parallel-stranded G-quadruplex DNA.,Wang Y, Patel DJ J Mol Biol. 1993 Dec 20;234(4):1171-83. PMID:8263919[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||