6jel: Difference between revisions
No edit summary |
No edit summary |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Structure of Phytolacca americana apo UGT2== | ||
<StructureSection load='6jel' size='340' side='right'caption='[[6jel]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6jel]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JEL FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jel OCA], [https://pdbe.org/6jel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jel RCSB], [https://www.ebi.ac.uk/pdbsum/6jel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jel ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B5MGN7_PHYAM B5MGN7_PHYAM] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The glycosylation of small hydrophobic compounds is catalyzed by uridine diphosphate glycosyltransferases (UGTs). Because glycosylation is an invaluable tool for improving the stability and water solubility of hydrophobic compounds, UGTs have attracted attention for their application in the food, cosmetics, and pharmaceutical industries. However, the ability of UGTs to accept and glycosylate a wide range of substrates is not clearly understood due to the existence of a large number of UGTs. PaGT2, a UGT from Phytolacca americana, can regioselectively glycosylate piceatannol but has low activity toward other stilbenoids. To elucidate the substrate specificity and catalytic mechanism, we determined the crystal structures of PaGT2 with and without substrates and performed molecular docking studies. The structures have revealed key residues involved in substrate recognition and suggest the presence of a nonconserved catalytic residue (His81) in addition to the highly conserved catalytic histidine in UGTs (His18). The role of the identified residues in substrate recognition and catalysis is elucidated with the mutational assay. Additionally, the structure-guided mutation of Cys142 to other residues, Ala, Phe, and Gln, allows PaGT2 to glycosylate resveratrol with high regioselectivity, which is negligibly glycosylated by the wild-type enzyme. These results provide a basis for tailoring an efficient glycosyltransferase. | |||
An Ambidextrous Polyphenol Glycosyltransferase PaGT2 from Phytolacca americana.,Maharjan R, Fukuda Y, Shimomura N, Nakayama T, Okimoto Y, Kawakami K, Nakayama T, Hamada H, Inoue T, Ozaki SI Biochemistry. 2020 Jul 14;59(27):2551-2561. doi: 10.1021/acs.biochem.0c00224., Epub 2020 Jul 2. PMID:32525309<ref>PMID:32525309</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6jel" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: Hamada | ==See Also== | ||
[[Category: | *[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]] | ||
[[Category: Nakayama | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Phytolacca americana]] | |||
[[Category: Fukuda Y]] | |||
[[Category: Hamada H]] | |||
[[Category: Inoue T]] | |||
[[Category: Maharjan R]] | |||
[[Category: Nakayama T]] | |||
[[Category: Ozaki S]] | |||
Latest revision as of 13:07, 22 November 2023
Structure of Phytolacca americana apo UGT2Structure of Phytolacca americana apo UGT2
Structural highlights
FunctionPublication Abstract from PubMedThe glycosylation of small hydrophobic compounds is catalyzed by uridine diphosphate glycosyltransferases (UGTs). Because glycosylation is an invaluable tool for improving the stability and water solubility of hydrophobic compounds, UGTs have attracted attention for their application in the food, cosmetics, and pharmaceutical industries. However, the ability of UGTs to accept and glycosylate a wide range of substrates is not clearly understood due to the existence of a large number of UGTs. PaGT2, a UGT from Phytolacca americana, can regioselectively glycosylate piceatannol but has low activity toward other stilbenoids. To elucidate the substrate specificity and catalytic mechanism, we determined the crystal structures of PaGT2 with and without substrates and performed molecular docking studies. The structures have revealed key residues involved in substrate recognition and suggest the presence of a nonconserved catalytic residue (His81) in addition to the highly conserved catalytic histidine in UGTs (His18). The role of the identified residues in substrate recognition and catalysis is elucidated with the mutational assay. Additionally, the structure-guided mutation of Cys142 to other residues, Ala, Phe, and Gln, allows PaGT2 to glycosylate resveratrol with high regioselectivity, which is negligibly glycosylated by the wild-type enzyme. These results provide a basis for tailoring an efficient glycosyltransferase. An Ambidextrous Polyphenol Glycosyltransferase PaGT2 from Phytolacca americana.,Maharjan R, Fukuda Y, Shimomura N, Nakayama T, Okimoto Y, Kawakami K, Nakayama T, Hamada H, Inoue T, Ozaki SI Biochemistry. 2020 Jul 14;59(27):2551-2561. doi: 10.1021/acs.biochem.0c00224., Epub 2020 Jul 2. PMID:32525309[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||