6j7h: Difference between revisions

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-'''Unreleased structure'''
-The entry 6j7h is ON HOLD
+==Crystal structure of blue fluorescent protein from metagenomic library==
+<StructureSection load='6j7h' size='340' side='right'caption='[[6j7h]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6j7h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J7H FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.309&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j7h OCA], [https://pdbe.org/6j7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j7h RCSB], [https://www.ebi.ac.uk/pdbsum/6j7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j7h ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/D6NKF4_9BACT D6NKF4_9BACT]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Fluorescent proteins, such as the green fluorescent protein, are used for detection of cellular components and events. However, green fluorescent protein and its derivatives have limited usage under anaerobic conditions and require a long maturation time. On the other hand, the NADPH-dependent blue fluorescent protein (BFP) without oxidative modification of residues is instantly functional in both aerobic and anaerobic systems. BFP proteins belong to a short-chain dehydrogenase/reductase (SDR) protein family, and their fluorescent property changes with reaction time in the presence of a substrate. With the aim of developing a better fluorescent reporter independent of redox state, we elucidated the crystal structure of a tetrameric mBFP from soil metagenomes with and without NADPH. Apart from the previously known regions, structure-guided mutational studies have identified several residues that contribute to the fluorescence of mBFP, including two aromatic residues (F97 and Y157) near the nicotinamide moiety of the bound NADPH. A single histidine mutation at Y157 (Y157H) has conferred more stabilized, time-independent fluorescence even in the presence of substrates. Furthermore, we discovered another SDR protein that can also emit blue fluorescence. These results open a new possibility for the development of BFP as a stable cellular reporter for widespread use, independent of subcellular environments.
-Authors: Seo, P.W., Kim, J.S.
+Structure-Guided Generation of a Redox-Independent Blue Fluorescent Protein from mBFP.,Seo PW, Jo ES, You SH, Cheong DE, Kim GJ, Kim JS J Mol Biol. 2019 Aug 9;431(17):3191-3202. doi: 10.1016/j.jmb.2019.06.005. Epub , 2019 Jun 13. PMID:31202883<ref>PMID:31202883</ref>
-Description: Crystal structure of blue fluorescent protein from metagenomic library
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Seo, P.W]]
+<div class="pdbe-citations 6j7h" style="background-color:#fffaf0;"></div>
-[[Category: Kim, J.S]]
+==See Also==
+*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Uncultured bacterium]]
+[[Category: Kim JS]]
+[[Category: Seo PW]]