6iu1: Difference between revisions

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-'''Unreleased structure'''
-The entry 6iu1 is ON HOLD
+==Peroxiredoxin from Pyrococcus horikoshii 0Cys mutant)==
+<StructureSection load='6iu1' size='340' side='right'caption='[[6iu1]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6iu1]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IU1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IU1 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.89&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iu1 OCA], [https://pdbe.org/6iu1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iu1 RCSB], [https://www.ebi.ac.uk/pdbsum/6iu1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iu1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TDXH_PYRHO TDXH_PYRHO]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Peroxiredoxins from Pyrococcus horikoshii (PhPrx) and Thermococcus kodakaraensis (TkPrx) are highly homologous proteins sharing 196 of the 216 residues. We previously reported a pentagonal ring-type decameric structure of PhPrx. Here, we present the crystal structure of TkPrx. Despite their homology, unlike PhPrx, the quaternary structure of TkPrx was found to be a dodecamer comprised of six homodimers arranged in a hexagonal ring-type assembly. The possibility of the redox-dependent conversion of the molecular assembly, which had been observed in PhPrx, was excluded for TkPrx based on the crystal structure of a mutant in which all of the cysteine residues were substituted with serine. The monomer structures of the dodecameric TkPrx and decameric PhPrx coincided well, but there was a slight difference in the relative orientation of the two domains. Molecular assembly of PhPrx and TkPrx in solution evaluated by gel-filtration chromatography was consistent with the crystallographic results. For both PhPrx and TkPrx, the gel-filtration elution volume slightly increased with a decrease in the protein concentration, suggesting the existence of an equilibrium state between the decameric/dodecameric ring and lower-order assembly. This structural assembly difference between highly homologous Prxs suggests a significant influence of quaternary structure on function, worthy of further exploration.
-Authors: Nakamura, T., Himiyama, T.
+Distinct molecular assembly of homologous peroxiredoxins from Pyrococcus horikoshii and Thermococcus kodakaraensis.,Himiyama T, Oshima M, Uegaki K, Nakamura T J Biochem. 2019 Feb 22. pii: 5362029. doi: 10.1093/jb/mvz013. PMID:30796432<ref>PMID:30796432</ref>
-Description: Peroxiredoxin from Pyrococcus horikoshii 0Cys mutant)
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Himiyama, T]]
+<div class="pdbe-citations 6iu1" style="background-color:#fffaf0;"></div>
-[[Category: Nakamura, T]]
+==See Also==
+*[[Peroxiredoxin 3D structures|Peroxiredoxin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Pyrococcus horikoshii OT3]]
+[[Category: Himiyama T]]
+[[Category: Nakamura T]]