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==Crystal structure of the minor ferredoxin from Thermosynechococcus elongatus== | |||
<StructureSection load='6iri' size='340' side='right'caption='[[6iri]], [[Resolution|resolution]] 1.38Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6iri]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IRI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IRI FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.38Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iri OCA], [https://pdbe.org/6iri PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iri RCSB], [https://www.ebi.ac.uk/pdbsum/6iri PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iri ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8DJI7_THEVB Q8DJI7_THEVB] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Photosynthetic [2Fe-2S] plant-type ferredoxins have a central role in electron transfer between the photosynthetic chain and various metabolic pathways. Several genes are coding for [2Fe2S] ferredoxins in cyanobacteria, with four in the thermophilic cyanobacterium Thermosynechococcus elongatus. The structure and functional properties of the major ferredoxin Fd1 are well known but data on the other ferredoxins are scarce. We report the structural and functional properties of a novel minor type ferredoxin, Fd2 of T. elongatus, homologous to Fed4 from Synechocystis sp. PCC 6803. Remarkably, the midpoint potential of Fd2, Em=-440mV, is lower than that of Fd1, Em=-372mV. However, while Fd2 can efficiently react with photosystem I or nitrite reductase, time-resolved spectroscopy shows that Fd2 has a very low capacity to reduce ferredoxin-NADP(+) oxidoreductase (FNR). These unique Fd2 properties are discussed in relation with its structure, solved at 1.38A resolution. The Fd2 structure significantly differs from other known ferredoxins structures in loop 2, N-terminal region, hydrogen bonding networks and surface charge distributions. UV-Vis, EPR, and Mid- and Far-IR data also show that the electronic properties of the [2Fe2S] cluster of Fd2 and its interaction with the protein differ from those of Fd1 both in the oxidized and reduced states. The structural analysis allows to propose that valine in the motif Cys53ValAsnCys56 of Fd2 and the specific orientation of Phe72, explain the electron transfer properties of Fd2. Strikingly, the nature of these residues correlates with different phylogenetic groups of cyanobacterial Fds. With its low redox potential and its discrimination against FNR, Fd2 exhibits a unique capacity to direct efficiently photosynthetic electrons to metabolic pathways not dependent on FNR. | |||
An alternative plant-like cyanobacterial ferredoxin with unprecedented structural and functional properties.,Motomura T, Zuccarello L, Setif P, Boussac A, Umena Y, Lemaire D, Tripathy JN, Sugiura M, Hienerwadel R, Shen JR, Berthomieu C Biochim Biophys Acta Bioenerg. 2019 Nov 1;1860(11):148084. doi:, 10.1016/j.bbabio.2019.148084. Epub 2019 Sep 12. PMID:31520614<ref>PMID:31520614</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6iri" style="background-color:#fffaf0;"></div> | ||
[[Category: Motomura | |||
[[Category: Umena | ==See Also== | ||
*[[Ferredoxin 3D structures|Ferredoxin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Thermosynechococcus vestitus BP-1]] | |||
[[Category: Motomura T]] | |||
[[Category: Shen JR]] | |||
[[Category: Umena Y]] | |||