6ahg: Difference between revisions
New page: '''Unreleased structure''' The entry 6ahg is ON HOLD Authors: Park, J.H., Kim, D.S., Park, Y.R., Lee, S.J. Description: Trimeric structure of concanavalin A from Canavalia ensiformis [... |
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The | ==Trimeric structure of concanavalin A from Canavalia ensiformis== | ||
<StructureSection load='6ahg' size='340' side='right'caption='[[6ahg]], [[Resolution|resolution]] 2.83Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6ahg]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AHG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AHG FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.83Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ahg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ahg OCA], [https://pdbe.org/6ahg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ahg RCSB], [https://www.ebi.ac.uk/pdbsum/6ahg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ahg ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CONA_CANEN CONA_CANEN] D-mannose specific lectin. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Concanavalin A (ConA) interacts with carbohydrates as a lectin, and recent reports proposed its application for detecting a diversity of viruses and pathogens. Structural studies have detailed the interaction between ConA and carbohydrates and the metal coordination environment with manganese and calcium ions (Mn-Ca-ConA). In this study, ConA was crystallized with a cadmium-containing precipitant, and the refined structure indicates that Mn(2)(+) was replaced by Cd(2)(+) (Cd-Ca-ConA). The structural comparison with ConA demonstrates that the metal-coordinated residues of Cd-Ca-ConA, that is Glu8, Asp10, Asn14, Asp19, and His24, do not have conformational shifts, but residues for sugar binding, including Arg228, Tyr100, and Leu99, reorient their side chains, slightly. Previous studies demonstrated that excess cadmium ions can coordinate with other residues, including Glu87 and Glu183, which were not coordinated with Cd(2)(+) in this study. The trimeric ConA in this study coordinated Cd(2)(+) with other residues, including Asp80 and Asp82, for the complex generation. The monomers does not have specific interaction near interface regions with the other monomer, but secondary cadmium coordinated with two aspartates (Asp80 and Asp82) from monomer 1 and one aspartate (Asp16) from monomer 2. This study demonstrated that complex generation was induced via coordination with secondary Cd(2)(+) and showed the application potential regarding the design of complex formation for specific interactions with target saccharides. | |||
Cadmium-substituted concanavalin A and its trimeric complexation.,Park YR, Kim DS, Lee DH, Kang HG, Park JH, Lee SJ J Microbiol Biotechnol. 2018 Oct 19. pii: 10.4014/jmb.1809.09027. doi:, 10.4014/jmb.1809.09027. PMID:30380822<ref>PMID:30380822</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6ahg" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: Park | *[[Concanavalin 3D structures|Concanavalin 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Canavalia ensiformis]] | |||
[[Category: Large Structures]] | |||
[[Category: Kim DS]] | |||
[[Category: Lee SJ]] | |||
[[Category: Park JH]] | |||
[[Category: Park YR]] | |||
Latest revision as of 12:30, 22 November 2023
Structural highlights
FunctionCONA_CANEN D-mannose specific lectin. Publication Abstract from PubMedConcanavalin A (ConA) interacts with carbohydrates as a lectin, and recent reports proposed its application for detecting a diversity of viruses and pathogens. Structural studies have detailed the interaction between ConA and carbohydrates and the metal coordination environment with manganese and calcium ions (Mn-Ca-ConA). In this study, ConA was crystallized with a cadmium-containing precipitant, and the refined structure indicates that Mn(2)(+) was replaced by Cd(2)(+) (Cd-Ca-ConA). The structural comparison with ConA demonstrates that the metal-coordinated residues of Cd-Ca-ConA, that is Glu8, Asp10, Asn14, Asp19, and His24, do not have conformational shifts, but residues for sugar binding, including Arg228, Tyr100, and Leu99, reorient their side chains, slightly. Previous studies demonstrated that excess cadmium ions can coordinate with other residues, including Glu87 and Glu183, which were not coordinated with Cd(2)(+) in this study. The trimeric ConA in this study coordinated Cd(2)(+) with other residues, including Asp80 and Asp82, for the complex generation. The monomers does not have specific interaction near interface regions with the other monomer, but secondary cadmium coordinated with two aspartates (Asp80 and Asp82) from monomer 1 and one aspartate (Asp16) from monomer 2. This study demonstrated that complex generation was induced via coordination with secondary Cd(2)(+) and showed the application potential regarding the design of complex formation for specific interactions with target saccharides. Cadmium-substituted concanavalin A and its trimeric complexation.,Park YR, Kim DS, Lee DH, Kang HG, Park JH, Lee SJ J Microbiol Biotechnol. 2018 Oct 19. pii: 10.4014/jmb.1809.09027. doi:, 10.4014/jmb.1809.09027. PMID:30380822[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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