6aby: Difference between revisions
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The | ==Crystal structure of citrate synthase (Msed_1522) from Metallosphaera sedula in complex with oxaloacetate== | ||
<StructureSection load='6aby' size='340' side='right'caption='[[6aby]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6aby]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Metallosphaera_sedula_DSM_5348 Metallosphaera sedula DSM 5348]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ABY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ABY FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6aby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aby OCA], [https://pdbe.org/6aby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6aby RCSB], [https://www.ebi.ac.uk/pdbsum/6aby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6aby ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A4YGX6_METS5 A4YGX6_METS5] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Metallosphaera sedula is a thermoacidophilic archaeon and has an incomplete TCA/glyoxylate cycle that is used for production of biosynthetic precursors of essential metabolites. Citrate synthase from M. sedula (MsCS) is an enzyme involved in the first step of the incomplete TCA/glyoxylate cycle by converting oxaloacetate and acetyl-CoA into citrate and coenzyme A. To elucidate the inhibition properties of MsCS, we determined its crystal structure at 1.7 A resolution. Like other Type-I CS, MsCS functions as a dimer and each monomer consists of two distinct domains, a large domain and a small domain. The oxaloacetate binding site locates at the cleft between the two domains, and the active site was more closed upon binding of the oxaloacetate substrate than binding of the citrate product. Interestingly, the inhibition kinetic analysis showed that, unlike other Type-I CSs, MsCS is non-competitively inhibited by NADH. Finally, amino acids and structural comparison of MsCS with other Type-II CSs, which were reported to be non-competitively inhibited by NADH, revealed that MsCS has quite unique NADH binding mode for non-competitive inhibition. | |||
Structural insights into the inhibition properties of archaeon citrate synthase from Metallosphaera sedula.,Lee SH, Son HF, Kim KJ PLoS One. 2019 Feb 22;14(2):e0212807. doi: 10.1371/journal.pone.0212807., eCollection 2019. PMID:30794680<ref>PMID:30794680</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Kim | <div class="pdbe-citations 6aby" style="background-color:#fffaf0;"></div> | ||
[[Category: Lee | |||
[[Category: Son | ==See Also== | ||
*[[Citrate Synthase 3D structures|Citrate Synthase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Metallosphaera sedula DSM 5348]] | |||
[[Category: Kim K-J]] | |||
[[Category: Lee S-H]] | |||
[[Category: Son H-F]] | |||
Latest revision as of 12:24, 22 November 2023
Crystal structure of citrate synthase (Msed_1522) from Metallosphaera sedula in complex with oxaloacetateCrystal structure of citrate synthase (Msed_1522) from Metallosphaera sedula in complex with oxaloacetate
Structural highlights
FunctionPublication Abstract from PubMedMetallosphaera sedula is a thermoacidophilic archaeon and has an incomplete TCA/glyoxylate cycle that is used for production of biosynthetic precursors of essential metabolites. Citrate synthase from M. sedula (MsCS) is an enzyme involved in the first step of the incomplete TCA/glyoxylate cycle by converting oxaloacetate and acetyl-CoA into citrate and coenzyme A. To elucidate the inhibition properties of MsCS, we determined its crystal structure at 1.7 A resolution. Like other Type-I CS, MsCS functions as a dimer and each monomer consists of two distinct domains, a large domain and a small domain. The oxaloacetate binding site locates at the cleft between the two domains, and the active site was more closed upon binding of the oxaloacetate substrate than binding of the citrate product. Interestingly, the inhibition kinetic analysis showed that, unlike other Type-I CSs, MsCS is non-competitively inhibited by NADH. Finally, amino acids and structural comparison of MsCS with other Type-II CSs, which were reported to be non-competitively inhibited by NADH, revealed that MsCS has quite unique NADH binding mode for non-competitive inhibition. Structural insights into the inhibition properties of archaeon citrate synthase from Metallosphaera sedula.,Lee SH, Son HF, Kim KJ PLoS One. 2019 Feb 22;14(2):e0212807. doi: 10.1371/journal.pone.0212807., eCollection 2019. PMID:30794680[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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