6a28: Difference between revisions
New page: '''Unreleased structure''' The entry 6a28 is ON HOLD Authors: Adachi, M., Shibazaki, C., Shimizu, R., Arai, S., Satoh, K., Narumi, I., Kuroki, R. Description: Crystal structure of PprA... |
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==Crystal structure of PprA W183R mutant form 2== | |||
<StructureSection load='6a28' size='340' side='right'caption='[[6a28]], [[Resolution|resolution]] 2.19Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6a28]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A28 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.193Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a28 OCA], [https://pdbe.org/6a28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a28 RCSB], [https://www.ebi.ac.uk/pdbsum/6a28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a28 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PPRA_DEIRA PPRA_DEIRA] dsDNA-binding protein that contributes to the ionizing radiation resistance of D.radiodurans. Plays a role in DNA repair and genome reconstitution, and is necessary for recovery from severe genomic fragmentation as a result of exposure to severe levels of ionizing radiation. In vitro, binds to double-stranded DNA carrying strand breaks and stimulates the DNA end-joining reaction catalyzed by DNA ligases. Thus, PprA plays a critical role in a non-homologous end-joining (NHEJ) pathway for the repair of radiation-induced DNA double-strands breaks. Cannot bind to dsDNA without strand breaks or single-stranded DNA.<ref>PMID:15454524</ref> <ref>PMID:15458422</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pleiotropic protein promoting DNA repair A (PprA) is a key protein facilitating the extreme radiation resistance of Deinococcus radiodurans. PprA is a unique protein to the genus Deinococcus and exists as an oligomer ranging from a tetramer to an approximately 100-mer depending on protein concentrations. Here, the X-ray crystal structure of PprA was determined to clarify how PprA confers radiation resistance. The tertiary structure of dimeric PprA was elucidated by using mutants obtained with random and site-directed mutagenesis methods (W183R and A139R); these mutants have disabled DNA binding and polymerization functions. Because the mutant A139R and W183R proteins have dimeric assemblies with 2 different interfaces (Interfaces 1 and 2), the linear and oligomerized PprA model was constructed as a left-handed face-to-face periodic screw structure. In addition, the linear structure in solution was confirmed by small-angle scattering experiments. The site-directed mutational analysis identified essential basic amino acids for DNA binding. These analytical data support the hypothesis that a complex assembly of PprA molecules, which are extended and have a screw structure, surrounds and stretches the DNA strand, acting as a novel guide to colocalize the DNA strands for efficient DNA repairs.-Adachi, M., Shimizu, R., Shibazaki, C., Satoh, K., Fujiwara, S., Arai, S., Narumi, I., Kuroki, R. Extended structure of pleiotropic DNA repair-promoting protein PprA from Deinococcus radiodurans. | |||
Extended structure of pleiotropic DNA repair-promoting protein PprA from Deinococcus radiodurans.,Adachi M, Shimizu R, Shibazaki C, Satoh K, Fujiwara S, Arai S, Narumi I, Kuroki R FASEB J. 2018 Nov 27:fj201801506R. doi: 10.1096/fj.201801506R. PMID:30481062<ref>PMID:30481062</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6a28" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Kuroki | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: Satoh | </StructureSection> | ||
[[Category: | [[Category: Deinococcus radiodurans R1]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Adachi M]] | |||
[[Category: Arai S]] | |||
[[Category: Kuroki R]] | |||
[[Category: Narumi I]] | |||
[[Category: Satoh K]] | |||
[[Category: Shibazaki C]] | |||
[[Category: Shimizu R]] | |||