5zz1: Difference between revisions
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<StructureSection load='5zz1' size='340' side='right'caption='[[5zz1]], [[Resolution|resolution]] 1.91Å' scene=''> | <StructureSection load='5zz1' size='340' side='right'caption='[[5zz1]], [[Resolution|resolution]] 1.91Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5zz1]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5zz1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycothermus_thermophilus Mycothermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZZ1 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3TR:3-AMINO-1,2,4-TRIAZOLE'>3TR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.91Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3TR:3-AMINO-1,2,4-TRIAZOLE'>3TR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zz1 OCA], [https://pdbe.org/5zz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zz1 RCSB], [https://www.ebi.ac.uk/pdbsum/5zz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zz1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/M4GGR7_9PEZI M4GGR7_9PEZI] Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.[RuleBase:RU004142] Serves to protect cells from the toxic effects of hydrogen peroxide.[PIRNR:PIRNR038927] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 19: | Line 19: | ||
</div> | </div> | ||
<div class="pdbe-citations 5zz1" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5zz1" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Catalase 3D structures|Catalase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Mycothermus thermophilus]] | ||
[[Category: McPherson | [[Category: McPherson MJ]] | ||
[[Category: Ogel | [[Category: Ogel ZB]] | ||
[[Category: Pearson | [[Category: Pearson AR]] | ||
[[Category: Trinh | [[Category: Trinh CH]] | ||
[[Category: | [[Category: Yuzugullu Karakus Y]] | ||
Latest revision as of 12:10, 22 November 2023
Probing the active center of catalase-phenol oxidase from Scytalidium thermophilumProbing the active center of catalase-phenol oxidase from Scytalidium thermophilum
Structural highlights
FunctionM4GGR7_9PEZI Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.[RuleBase:RU004142] Serves to protect cells from the toxic effects of hydrogen peroxide.[PIRNR:PIRNR038927] Publication Abstract from PubMedThe catalase from Scytalidium thermophilum is a homotetramer containing a heme d in each active site. Although the enzyme has a classical monofunctional catalase fold, it also possesses oxidase activity towards a number of small organics, including catechol and phenol. In order to further investigate this, the crystal structure of the complex of the catalase with the classical catalase inhibitor 3-amino-1,2,4-triazole (3TR) was determined at 1.95 A resolution. Surprisingly, no binding to the heme site was observed; instead, 3TR occupies a binding site corresponding to the NADPH-binding pocket in mammalian catalases at the entrance to a lateral channel leading to the heme. Kinetic analysis of site-directed mutants supports the assignment of this pocket as the binding site for oxidase substrates. Identification of the site of oxidase substrate binding in Scytalidium thermophilum catalase.,Yuzugullu Karakus Y, Goc G, Balci S, Yorke BA, Trinh CH, McPherson MJ, Pearson AR Acta Crystallogr D Struct Biol. 2018 Oct 1;74(Pt 10):979-985. doi:, 10.1107/S2059798318010628. Epub 2018 Oct 2. PMID:30289408[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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