5zh6: Difference between revisions
New page: '''Unreleased structure''' The entry 5zh6 is ON HOLD Authors: Yang, R.Q., Chen, Y.L., Jin, T.C., Cao, M.J. Description: Crystal structure of Parvalbumin SPV-II of Mustelus griseus [[Ca... |
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==Crystal structure of Parvalbumin SPV-II of Mustelus griseus== | |||
<StructureSection load='5zh6' size='340' side='right'caption='[[5zh6]], [[Resolution|resolution]] 1.54Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5zh6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mustelus_griseus Mustelus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZH6 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zh6 OCA], [https://pdbe.org/5zh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zh6 RCSB], [https://www.ebi.ac.uk/pdbsum/5zh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zh6 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A3F2YLV2_MUSGR A0A3F2YLV2_MUSGR] In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.[RuleBase:RU368048] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Fish play important roles in human nutrition and health, but also trigger allergic reactions in some population. Parvalbumin (PV) represents the major allergen of fish. While IgE cross-reactivity to PV in various bony fish species has been well characterized, little information is available about allergens in cartilaginous fish. In this study, two shark PV isoforms (named as SPV-I and SPV-II) from Mustelus griseus were purified. Their identities were further confirmed by mass spectroscopic analysis. IgE immunoblot analysis showed that sera from fish-allergic patients reacted to both SPV-I and SPV-II, but the majority of sera reacted more intensely to SPV-I than SPV-II. Thermal denaturation monitored by CD spectrum showed that both of the SPV allergens are highly thermostable. SPV-I maintained its IgE-binding capability after heat denaturation, while the IgE-binding capability of SPV-II was reduced. The results of crystal structure showed that SPV-I and SPV-II were similar in their overall tertiary structure, but their amino acid sequences shared lower similarities, indicating that the differences in the IgE-binding capabilities of SPV-I and SPV-II might be due to differential antigen epitopes in these two isoforms. | |||
Purification, Characterization, and Crystal Structure of Parvalbumins, the Major Allergens in Mustelus griseus.,Yang RQ, Chen YL, Chen F, Wang H, Zhang Q, Liu GM, Jin T, Cao MJ J Agric Food Chem. 2018 Jul 17. doi: 10.1021/acs.jafc.8b01889. PMID:29969026<ref>PMID:29969026</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5zh6" style="background-color:#fffaf0;"></div> | ||
[[Category: Chen | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Parvalbumin|Parvalbumin]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mustelus griseus]] | |||
[[Category: Cao MJ]] | |||
[[Category: Chen YL]] | |||
[[Category: Jin TC]] | |||
[[Category: Yang RQ]] | |||