5xft: Difference between revisions

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-'''Unreleased structure'''
-The entry 5xft is ON HOLD
+==Crystal structure of Chlamydomonas reinhardtii dehydroascorbate reductase==
+<StructureSection load='5xft' size='340' side='right'caption='[[5xft]], [[Resolution|resolution]] 2.46&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5xft]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XFT FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.46&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xft OCA], [https://pdbe.org/5xft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xft RCSB], [https://www.ebi.ac.uk/pdbsum/5xft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xft ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A8I0K9_CHLRE A8I0K9_CHLRE]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Dehydroascorbate reductase (DHAR) is a key enzyme for glutathione (GSH)-dependent reduction of dehydroascorbate (DHA) to recycled ascorbate (AsA) in plants, and plays a major role against the toxicity of reactive oxygen species (ROS). Previously, we proposed that the increase of AsA regeneration via enhanced DHAR activity modulates the ascorbate-glutathione cycle activity against photooxidative stress in Chlamydomonas reinhardtii. In the present work, we use site-directed mutagenesis and crystal structure analysis to elucidate the molecular basis of how C. reinhardtii DHAR (CrDHAR1) is involved in the detoxification mechanisms. Mutagenesis data show that the D21A, D21N and C22A mutations result in severe loss of the enzyme's function, suggesting crucial roles of Asp-21 and Cys-22 in substrate binding and catalysis. The mutant K11A also exhibits reduced redox activity ( approximately 50%). The crystal structure of apo CrDHAR1 further provides insights into the proposed mechanism centering on the strictly conserved Cys-22, which is suggested to initiate the redox reactions of DHA and GSH. Furthermore, in vitro oxidation of the recombinant CrDHAR1 in the presence of 1 mM H2O2 has minor effects on the Km for the substrates but significantly reduces the kcat. The enzyme's activity and its mRNA abundance in the C. reinhardtii cells are increased by treatment with 0.2-1 mM H2O2 but decreased when H2O2 is &gt;/= 1.5 mM. The latter decrease is accompanied by oxidative damage and lower AsA concentrations. These biochemical and physiological data provide new insights into the catalytic mechanism of CrDHAR1, which protects the C. reinhardtii cells from oxidative stress-induced toxicity.
-Authors: Chang, H.Y., Ko, T.P., Huang, K.F.
+Enzymatic characterization and crystal structure analysis of Chlamydomonas reinhardtii dehydroascorbate reductase and their implications for oxidative stress.,Chang HY, Lin ST, Ko TP, Wu SM, Lin TH, Chang YC, Huang KF, Lee TM Plant Physiol Biochem. 2017 Nov;120:144-155. doi: 10.1016/j.plaphy.2017.09.026., Epub 2017 Oct 3. PMID:29028546<ref>PMID:29028546</ref>
-Description: Crystal structure of Chlamydomonas reinhardtii dehydroascorbate reductase
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Ko, T.P]]
+<div class="pdbe-citations 5xft" style="background-color:#fffaf0;"></div>
-[[Category: Chang, H.Y]]
+== References ==
-[[Category: Huang, K.F]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Chlamydomonas reinhardtii]]
+[[Category: Large Structures]]
+[[Category: Chang HY]]
+[[Category: Huang KF]]
+[[Category: Ko TP]]