5xce: Difference between revisions
New page: '''Unreleased structure''' The entry 5xce is ON HOLD until Paper Publication Authors: Srivastava, V.K., Yadav, R., Tomar, P., Gourinath, S., Datta, S. Description: Crystal structure of... |
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==Crystal structure of Wild type Vps29 from Entamoeba histolytica== | |||
<StructureSection load='5xce' size='340' side='right'caption='[[5xce]], [[Resolution|resolution]] 1.86Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5xce]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XCE FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xce OCA], [https://pdbe.org/5xce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xce RCSB], [https://www.ebi.ac.uk/pdbsum/5xce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xce ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9BI08_ENTHI Q9BI08_ENTHI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Vps29 is the smallest subunit of retromer complex with metallo-phosphatase fold. Although the role of metal in Vps29 is in quest, its metal binding mutants has been reported to affect the localization of the retromer complex in human cells. In this study, we report the structural and thermodynamic consequences of these mutations in Vps29 from the protozoan parasite, Entamoeba histolytica (EhVps29). EhVps29 is a zinc binding protein as revealed by X-ray crystallography and isothermal titration calorimetry. The metal binding pocket of EhVps29 exhibits marked differences in its 3-dimensional architecture and metal coordination in comparison to its human homologs and other metallo-phosphatases. Alanine substitutions of the metal-coordinating residues showed significant alteration in the binding affinity of EhVps29 for zinc. We also determined the crystal structures of metal binding defective mutants (D62A and D62A/H86A) of EhVps29. Based on our results, we propose that the metal atoms or the bound water molecules in the metal binding site are important for maintaining the structural integrity of the protein. Further cellular studies in the amoebic trophozoites showed that the overexpression of wild type EhVps29 leads to reduction in intracellular cysteine protease activity suggesting its crucial role in secretion of the proteases. This article is protected by copyright. All rights reserved. | |||
Structural and thermodynamic characterization of metal binding in Vps29 from Entamoeba histolytica: Implication in retromer function.,Srivastava VK, Yadav R, Natsuki W, Tomar P, Mukherjee M, Gourinath S, Nakada-Tsukui K, Nozaki T, Datta S Mol Microbiol. 2017 Sep 12. doi: 10.1111/mmi.13836. PMID:28898487<ref>PMID:28898487</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Datta | <div class="pdbe-citations 5xce" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Vacuolar protein sorting-associated protein 3D structures|Vacuolar protein sorting-associated protein 3D structures]] | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Entamoeba histolytica]] | |||
[[Category: Large Structures]] | |||
[[Category: Datta S]] | |||
[[Category: Gourinath S]] | |||
[[Category: Srivastava VK]] | |||
[[Category: Tomar P]] | |||
[[Category: Yadav R]] | |||