5x9r: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''Unreleased structure'''
-The entry 5x9r is ON HOLD
+==Structural insights into the elevator-like mechanism of the sodium/citrate symporter CitS==
+<StructureSection load='5x9r' size='340' side='right'caption='[[5x9r]], [[Resolution|resolution]] 3.98&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5x9r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X9R FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.98&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x9r OCA], [https://pdbe.org/5x9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x9r RCSB], [https://www.ebi.ac.uk/pdbsum/5x9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x9r ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CITN_KLEPN CITN_KLEPN] Uptake of citrate across the boundary membrane with the concomitant uptake of a sodium ion (symport system).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The sodium-dependent citrate transporter of Klebsiella pneumoniae (KpCitS) belongs to the 2-hydroxycarboxylate transporter (2-HCT) family and allows the cell to use citrate as sole carbon and energy source in anaerobic conditions. Here we present crystal structures of KpCitS in citrate-bound outward-facing, citrate-bound asymmetric, and citrate-free inward-facing state. The structures reveal that the KpCitS dimerization domain remains stationary throughout the transport cycle due to a hydrogen bond network as well as extensive hydrophobic interactions. In contrast, its transport domain undergoes a ~35 degrees rigid-body rotation and a ~17 A translocation perpendicular to the membrane to expose the substrate-binding site alternately to either side of the membrane. Furthermore, homology models of two other 2-HCT proteins based on the KpCitS structure offer structural insights into their differences in substrate specificity at a molecular level. On the basis of our results and previous biochemical data, we propose that the activity of the 2-HCT CitS involves an elevator-like movement in which the transport domain itself traverses the lipid bilayer, carrying the substrate into the cell in a sodium-dependent manner.
-Authors:
+Structural insights into the elevator-like mechanism of the sodium/citrate symporter CitS.,Kim JW, Kim S, Kim S, Lee H, Lee JO, Jin MS Sci Rep. 2017 May 31;7(1):2548. doi: 10.1038/s41598-017-02794-x. PMID:28566738<ref>PMID:28566738</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 5x9r" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Symporter 3D structures|Symporter 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Klebsiella pneumoniae]]
+[[Category: Large Structures]]
+[[Category: Jin MS]]
+[[Category: Kim JW]]
+[[Category: Kim S]]
+[[Category: Lee H]]
+[[Category: Lee J-O]]