5nm5: Difference between revisions

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-'''Unreleased structure'''
-The entry 5nm5 is ON HOLD
+==Tubulin Darpin room-temperature structure in complex with Colchicine determined by serial millisecond crystallography==
+<StructureSection load='5nm5' size='340' side='right'caption='[[5nm5]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5nm5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NM5 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=LOC:N-[(7S)-1,2,3,10-TETRAMETHOXY-9-OXO-6,7-DIHYDRO-5H-BENZO[D]HEPTALEN-7-YL]ETHANAMIDE'>LOC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nm5 OCA], [https://pdbe.org/5nm5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nm5 RCSB], [https://www.ebi.ac.uk/pdbsum/5nm5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nm5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Historically, room-temperature structure determination was succeeded by cryo-crystallography to mitigate radiation damage. Here, we demonstrate that serial millisecond crystallography at a synchrotron beamline equipped with high-viscosity injector and high frame-rate detector allows typical crystallographic experiments to be performed at room-temperature. Using a crystal scanning approach, we determine the high-resolution structure of the radiation sensitive molybdenum storage protein, demonstrate soaking of the drug colchicine into tubulin and native sulfur phasing of the human G protein-coupled adenosine receptor. Serial crystallographic data for molecular replacement already converges in 1,000-10,000 diffraction patterns, which we collected in 3 to maximally 82 minutes. Compared with serial data we collected at a free-electron laser, the synchrotron data are of slightly lower resolution, however fewer diffraction patterns are needed for de novo phasing. Overall, the data we collected by room-temperature serial crystallography are of comparable quality to cryo-crystallographic data and can be routinely collected at synchrotrons.Serial crystallography was developed for protein crystal data collection with X-ray free-electron lasers. Here the authors present several examples which show that serial crystallography using high-viscosity injectors can also be routinely employed for room-temperature data collection at synchrotrons.
-Authors: Weinert, T., Olieric, N., James, D., Gashi, D., Nogly, P., Jaeger, K., Steinmetz, M.O., Standfuss, J.
+Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons.,Weinert T, Olieric N, Cheng R, Brunle S, James D, Ozerov D, Gashi D, Vera L, Marsh M, Jaeger K, Dworkowski F, Panepucci E, Basu S, Skopintsev P, Dore AS, Geng T, Cooke RM, Liang M, Prota AE, Panneels V, Nogly P, Ermler U, Schertler G, Hennig M, Steinmetz MO, Wang M, Standfuss J Nat Commun. 2017 Sep 14;8(1):542. doi: 10.1038/s41467-017-00630-4. PMID:28912485<ref>PMID:28912485</ref>
-Description: Tubulin Darpin room-temperature structure in complex with Colchicine determined by serial millisecond crystallography
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Nogly, P]]
+<div class="pdbe-citations 5nm5" style="background-color:#fffaf0;"></div>
-[[Category: Olieric, N]]
+== References ==
-[[Category: James, D]]
+<references/>
-[[Category: Weinert, T]]
+__TOC__
-[[Category: Jaeger, K]]
+</StructureSection>
-[[Category: Standfuss, J]]
+[[Category: Bos taurus]]
-[[Category: Steinmetz, M.O]]
+[[Category: Large Structures]]
-[[Category: Gashi, D]]
+[[Category: Synthetic construct]]
+[[Category: Gashi D]]
+[[Category: Jaeger K]]
+[[Category: James D]]
+[[Category: Nogly P]]
+[[Category: Olieric N]]
+[[Category: Standfuss J]]
+[[Category: Steinmetz MO]]
+[[Category: Weinert T]]