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New page: '''Unreleased structure''' The entry 5nil is ON HOLD Authors: Fitzpatrick, A.W.P., Llabres, S., Neuberger, A., Blaza, J.N., Bai, X.-C., Okada, U., Murakami, S., van Veen, H.W., Zacharia... |
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The entry | ==Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump-MacB section== | ||
<SX load='5nil' size='340' side='right' viewer='molstar' caption='[[5nil]], [[Resolution|resolution]] 5.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5nil]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NIL FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nil OCA], [https://pdbe.org/5nil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nil RCSB], [https://www.ebi.ac.uk/pdbsum/5nil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nil ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TOLC_ECOLI TOLC_ECOLI] Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.<ref>PMID:6337123</ref> <ref>PMID:11274125</ref> <ref>PMID:15228545</ref> <ref>PMID:18955484</ref> <ref>PMID:23176499</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The MacA-MacB-TolC assembly of Escherichia coli is a transmembrane machine that spans the cell envelope and actively extrudes substrates, including macrolide antibiotics and polypeptide virulence factors. These transport processes are energized by the ATPase MacB, a member of the ATP-binding cassette (ABC) superfamily. We present an electron cryo-microscopy structure of the ABC-type tripartite assembly at near-atomic resolution. A hexamer of the periplasmic protein MacA bridges between a TolC trimer in the outer membrane and a MacB dimer in the inner membrane, generating a quaternary structure with a central channel for substrate translocation. A gating ring found in MacA is proposed to act as a one-way valve in substrate transport. The MacB structure features an atypical transmembrane domain with a closely packed dimer interface and a periplasmic opening that is the likely portal for substrate entry from the periplasm, with subsequent displacement through an allosteric transport mechanism. | |||
Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump.,Fitzpatrick AWP, Llabres S, Neuberger A, Blaza JN, Bai XC, Okada U, Murakami S, van Veen HW, Zachariae U, Scheres SHW, Luisi BF, Du D Nat Microbiol. 2017 May 15;2:17070. doi: 10.1038/nmicrobiol.2017.70. PMID:28504659<ref>PMID:28504659</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5nil" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </SX> | ||
[[Category: | [[Category: Escherichia coli K-12]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Bai X-C]] | ||
[[Category: Okada | [[Category: Blaza JN]] | ||
[[Category: | [[Category: Du D]] | ||
[[Category: | [[Category: Fitzpatrick AWP]] | ||
[[Category: | [[Category: Llabres S]] | ||
[[Category: Luisi BF]] | |||
[[Category: Murakami S]] | |||
[[Category: Neuberger A]] | |||
[[Category: Okada U]] | |||
[[Category: Scheres SHW]] | |||
[[Category: Zachariae U]] | |||
[[Category: Van Veen HW]] | |||
Latest revision as of 16:00, 15 November 2023
Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump-MacB sectionStructure of the MacAB-TolC ABC-type tripartite multidrug efflux pump-MacB section
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