5nd8: Difference between revisions
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==Hibernating ribosome from Staphylococcus aureus (Unrotated state)== | |||
<SX load='5nd8' size='340' side='right' viewer='molstar' caption='[[5nd8]], [[Resolution|resolution]] 3.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5nd8]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ND8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ND8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nd8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nd8 OCA], [https://pdbe.org/5nd8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nd8 RCSB], [https://www.ebi.ac.uk/pdbsum/5nd8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nd8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RS2_STAA8 RS2_STAA8] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In bacteria, ribosomal hibernation shuts down translation as a response to stress, through reversible binding of stress-induced proteins to ribosomes. This process typically involves the formation of 100S ribosome dimers. Here, we present the structures of hibernating ribosomes from human pathogen Staphylococcus aureus containing a long variant of the hibernation-promoting factor (SaHPF) that we solved using cryo-electron microscopy. Our reconstructions reveal that the N-terminal domain (NTD) of SaHPF binds to the 30S subunit as observed for shorter variants of HPF in other species. The C-terminal domain (CTD) of SaHPF protrudes out of each ribosome in order to mediate dimerization. Using NMR, we characterized the interactions at the CTD-dimer interface. Secondary interactions are provided by helix 26 of the 16S ribosomal RNA We also show that ribosomes in the 100S particle adopt both rotated and unrotated conformations. Overall, our work illustrates a specific mode of ribosome dimerization by long HPF, a finding that may help improve the selectivity of antimicrobials. | |||
Structures and dynamics of hibernating ribosomes from Staphylococcus aureus mediated by intermolecular interactions of HPF.,Khusainov I, Vicens Q, Ayupov R, Usachev K, Myasnikov A, Simonetti A, Validov S, Kieffer B, Yusupova G, Yusupov M, Hashem Y EMBO J. 2017 Jul 14;36(14):2073-2087. doi: 10.15252/embj.201696105. Epub 2017 Jun, 23. PMID:28645916<ref>PMID:28645916</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5nd8" style="background-color:#fffaf0;"></div> | ||
[[Category: Hashem | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Ribosome 3D structures|Ribosome 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </SX> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Staphylococcus aureus subsp. aureus NCTC 8325]] | ||
[[Category: | [[Category: Ayupov R]] | ||
[[Category: Hashem Y]] | |||
[[Category: Khusainov I]] | |||
[[Category: Kieffer B]] | |||
[[Category: Myasnikov A]] | |||
[[Category: Simonetti A]] | |||
[[Category: Usachev K]] | |||
[[Category: Validov S]] | |||
[[Category: Vicens Q]] | |||
[[Category: Yusupov M]] | |||
[[Category: Yusupova G]] | |||
Latest revision as of 15:48, 15 November 2023
Hibernating ribosome from Staphylococcus aureus (Unrotated state)Hibernating ribosome from Staphylococcus aureus (Unrotated state)
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