4uts: Difference between revisions

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-'''Unreleased structure'''
-The entry 4uts is ON HOLD  until Paper Publication
+==Room temperature crystal structure of the fast switching M159T mutant of fluorescent protein Dronpa==
+<StructureSection load='4uts' size='340' side='right'caption='[[4uts]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4uts]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._SC22 Echinophyllia sp. SC22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UTS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UTS FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uts OCA], [https://pdbe.org/4uts PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uts RCSB], [https://www.ebi.ac.uk/pdbsum/4uts PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uts ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The fluorescent protein Dronpa undergoes reversible photoswitching reactions between the bright "on" and dark "off" states via photoisomerization and proton transfer reactions. We report the room temperature crystal structure of the fast switching Met159Thr mutant of Dronpa at 2.0-A resolution in the bright on state. Structural differences with the wild type include shifted backbone positions of strand beta8 containing Thr159 as well as an altered A-C dimer interface involving strands beta7, beta8, beta10, and beta11. The Met159Thr mutation increases the cavity volume for the p-hydroxybenzylidene-imidazolinone chromophore as a result of both the side chain difference and the backbone positional differences.
-Authors: Kaucikas, M., Fitzpatrick, A., Bryan, E., Struve, A., Henning, R., Kosheleva, I., Srajer, V., van Thor, J.J.
+Room temperature crystal structure of the fast switching M159T mutant of the fluorescent protein dronpa.,Kaucikas M, Fitzpatrick A, Bryan E, Struve A, Henning R, Kosheleva I, Srajer V, Groenhof G, Van Thor JJ Proteins. 2015 Mar;83(3):397-402. doi: 10.1002/prot.24742. Epub 2015 Jan 13. PMID:25524427<ref>PMID:25524427</ref>
-Description: Room temperature crystal structure of the fast switching M159T mutant of fluorescent protein Dronpa
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Struve, A]]
+<div class="pdbe-citations 4uts" style="background-color:#fffaf0;"></div>
-[[Category: Kaucikas, M]]
-[[Category: Kosheleva, I]]
+==See Also==
-[[Category: Van Thor, J.J]]
+*[[Dronpa|Dronpa]]
-[[Category: Bryan, E]]
+== References ==
-[[Category: Srajer, V]]
+<references/>
-[[Category: Fitzpatrick, A]]
+__TOC__
-[[Category: Henning, R]]
+</StructureSection>
+[[Category: Echinophyllia sp. SC22]]
+[[Category: Large Structures]]
+[[Category: Bryan E]]
+[[Category: Fitzpatrick A]]
+[[Category: Henning R]]
+[[Category: Kaucikas M]]
+[[Category: Kosheleva I]]
+[[Category: Srajer V]]
+[[Category: Struve A]]
+[[Category: Van Thor JJ]]