5myd: Difference between revisions
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==Convergent evolution involving dimeric and trimeric dUTPases in signalling.== | ==Convergent evolution involving dimeric and trimeric dUTPases in signalling.== | ||
<StructureSection load='5myd' size='340' side='right' caption='[[5myd]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='5myd' size='340' side='right'caption='[[5myd]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5myd]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MYD OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5myd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_sp._HMSC068C09 Staphylococcus sp. HMSC068C09]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MYD FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DUP:2-DEOXYURIDINE+5-ALPHA,BETA-IMIDO-TRIPHOSPHATE'>DUP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5myd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5myd OCA], [https://pdbe.org/5myd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5myd RCSB], [https://www.ebi.ac.uk/pdbsum/5myd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5myd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A2D0TC86_9STAP A0A2D0TC86_9STAP] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 5myd" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5myd" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[DUTPase 3D structures|DUTPase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Alite | [[Category: Staphylococcus sp. HMSC068C09]] | ||
[[Category: Bowring | [[Category: Alite C]] | ||
[[Category: Ciges-Tomas | [[Category: Bowring J]] | ||
[[Category: Donderis | [[Category: Ciges-Tomas JR]] | ||
[[Category: Maiques | [[Category: Donderis J]] | ||
[[Category: Marina | [[Category: Maiques E]] | ||
[[Category: Mehmedov | [[Category: Marina A]] | ||
[[Category: Penades | [[Category: Mehmedov I]] | ||
[[Category: Tormo-Mas | [[Category: Penades JR]] | ||
[[Category: Tormo-Mas MA]] | |||
Latest revision as of 20:53, 8 November 2023
Convergent evolution involving dimeric and trimeric dUTPases in signalling.Convergent evolution involving dimeric and trimeric dUTPases in signalling.
Structural highlights
FunctionPublication Abstract from PubMedThe dUTPase (Dut) enzymes, encoded by almost all free-living organisms and some viruses, prevent the misincorporation of uracil into DNA. We previously proposed that trimeric Duts are regulatory proteins involved in different cellular processes; including the phage-mediated transfer of the Staphylococcus aureus pathogenicity island SaPIbov1. Recently, it has been shown that the structurally unrelated dimeric Dut encoded by phage varphiNM1 is similarly able to mobilize SaPIbov1, suggesting dimeric Duts could also be regulatory proteins. How this is accomplished remains unsolved. Here, using in vivo, biochemical and structural approaches, we provide insights into the signaling mechanism used by the dimeric Duts to induce the SaPIbov1 cycle. As reported for the trimeric Duts, dimeric Duts contain an extremely variable region, here named domain VI, which is involved in the regulatory capacity of these enzymes. Remarkably, our results also show that the dimeric Dut signaling mechanism is modulated by dUTP, as with the trimeric Duts. Overall, our results demonstrate that although unrelated both in sequence and structure, dimeric and trimeric Duts control SaPI transfer by analogous mechanisms, representing a fascinating example of convergent evolution. This conserved mode of action highlights the biological significance of Duts as regulatory molecules. Convergent evolution involving dimeric and trimeric dUTPases in pathogenicity island mobilization.,Donderis J, Bowring J, Maiques E, Ciges-Tomas JR, Alite C, Mehmedov I, Tormo-Mas MA, Penades JR, Marina A PLoS Pathog. 2017 Sep 11;13(9):e1006581. doi: 10.1371/journal.ppat.1006581. PMID:28892519[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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