|
|
| Line 3: |
Line 3: |
| <SX load='5mmm' size='340' side='right' viewer='molstar' caption='[[5mmm]], [[Resolution|resolution]] 3.40Å' scene=''> | | <SX load='5mmm' size='340' side='right' viewer='molstar' caption='[[5mmm]], [[Resolution|resolution]] 3.40Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5mmm]] is a 60 chain structure with sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MMM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MMM FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5mmm]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MMM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MMM FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> |
| <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mmm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mmm OCA], [http://pdbe.org/5mmm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mmm RCSB], [http://www.ebi.ac.uk/pdbsum/5mmm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mmm ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mmm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mmm OCA], [https://pdbe.org/5mmm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mmm RCSB], [https://www.ebi.ac.uk/pdbsum/5mmm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mmm ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/RK20_SPIOL RK20_SPIOL]] Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit (By similarity).[HAMAP-Rule:MF_00382] [[http://www.uniprot.org/uniprot/RK11_SPIOL RK11_SPIOL]] This protein binds directly to 23S ribosomal RNA (By similarity). [[http://www.uniprot.org/uniprot/RR8_SPIOL RR8_SPIOL]] One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit (By similarity).[HAMAP-Rule:MF_01302] [[http://www.uniprot.org/uniprot/RR19_SPIOL RR19_SPIOL]] This protein binds directly to 16S ribosomal RNA.<ref>PMID:10874039</ref> [[http://www.uniprot.org/uniprot/RK21_SPIOL RK21_SPIOL]] This protein binds to 23S ribosomal RNA in the presence of protein L20 (By similarity). [[http://www.uniprot.org/uniprot/RK19_SPIOL RK19_SPIOL]] Located at the 30S-50S ribosomal subunit interface and binds directly to 23S ribosomal RNA (By similarity).[:] [[http://www.uniprot.org/uniprot/RK22_SPIOL RK22_SPIOL]] This protein binds specifically to 23S rRNA (By similarity). The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome (By similarity). Binds an erythromycin derivative added to the 50S subunit. [[http://www.uniprot.org/uniprot/RR4_SPIOL RR4_SPIOL]] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit (By similarity).[HAMAP-Rule:MF_01306] With S5 and S12 plays an important role in translational accuracy (By similarity).[HAMAP-Rule:MF_01306] [[http://www.uniprot.org/uniprot/PRSP1_SPIOL PRSP1_SPIOL]] A ribosome-binding factor that may be involved in an unknown stress response. Modeling onto the 70S spinach chloroplast ribosome and its position in the E.coli 70S ribosome suggests it binds in the decoding region of the 30S ribosomal subunit, precluding the binding of tRNA to the ribosome. Its position is incompatible with translation. Upon expression in E.coli binds to 30S and 70S ribosomes, decreases binding of tRNA(fMet). Stabilizes 70S ribosomes against dissociation. May be recycled by the combined action of ribosome-recycling factor (RRF) and EF-G.<ref>PMID:19965869</ref> [[http://www.uniprot.org/uniprot/RK14_SPIOL RK14_SPIOL]] Binds to 23S rRNA (By similarity). [[http://www.uniprot.org/uniprot/RRP3_SPIOL RRP3_SPIOL]] One of the plastid-specific ribosomal proteins. [[http://www.uniprot.org/uniprot/RR12_SPIOL RR12_SPIOL]] With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits (By similarity).[HAMAP-Rule:MF_00403_B] [[http://www.uniprot.org/uniprot/RK23_SPIOL RK23_SPIOL]] Binds to 23S rRNA (By similarity). Located at the polypeptide exit tunnel on the outside of the subunit. [[http://www.uniprot.org/uniprot/RR14_SPIOL RR14_SPIOL]] Binds 16S rRNA, required for the assembly of 30S particles (By similarity).[:] [[http://www.uniprot.org/uniprot/RRP2_SPIOL RRP2_SPIOL]] May have a role in the recruitment of stored chloroplast mRNAs for active protein synthesis.<ref>PMID:12605670</ref> [[http://www.uniprot.org/uniprot/RR5_SPIOL RR5_SPIOL]] Binds directly to 16S ribosomal RNA. Involved in spectinomycin and neamine resistance and streptomycin independence.[:] [[http://www.uniprot.org/uniprot/RK34_SPIOL RK34_SPIOL]] This protein binds directly to 23S ribosomal RNA (By similarity). [[http://www.uniprot.org/uniprot/RR7_SPIOL RR7_SPIOL]] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit (By similarity).[HAMAP-Rule:MF_00480] | | [https://www.uniprot.org/uniprot/RK31_SPIOL RK31_SPIOL] Component of the chloroplast ribosome (chloro-ribosome), a dedicated translation machinery responsible for the synthesis of chloroplast genome-encoded proteins, including proteins of the transcription and translation machinery and components of the photosynthetic apparatus.<ref>PMID:10874046</ref> <ref>PMID:28007896</ref> |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 19: |
Line 19: |
| </div> | | </div> |
| <div class="pdbe-citations 5mmm" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5mmm" style="background-color:#fffaf0;"></div> |
| | |
| | ==See Also== |
| | *[[Ribosome 3D structures|Ribosome 3D structures]] |
| == References == | | == References == |
| <references/> | | <references/> |
| Line 25: |
Line 28: |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Spinacia oleracea]] | | [[Category: Spinacia oleracea]] |
| [[Category: Ban, N]] | | [[Category: Ban N]] |
| [[Category: Bieri, P]] | | [[Category: Bieri P]] |
| [[Category: Boehringer, D]] | | [[Category: Boehringer D]] |
| [[Category: Leibundgut, M]] | | [[Category: Leibundgut M]] |
| [[Category: Saurer, M]] | | [[Category: Saurer M]] |
| [[Category: Chloroplast]]
| |
| [[Category: Cryo-em]]
| |
| [[Category: Ribosome]]
| |
| [[Category: Translation]]
| |