5cf3: Difference between revisions

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-'''Unreleased structure'''
-The entry 5cf3 is ON HOLD  until Paper Publication
+==Crystal structures of Bbp from Staphylococcus aureus==
+<StructureSection load='5cf3' size='340' side='right'caption='[[5cf3]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5cf3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CF3 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.031&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cf3 OCA], [https://pdbe.org/5cf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cf3 RCSB], [https://www.ebi.ac.uk/pdbsum/5cf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cf3 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/BBP_STAAU BBP_STAAU] Specifically interacts with bone sialoprotein (BSP), a glycoprotein of bone and dentin extracellular matrix. Could contribute to staphylococcal osteomyelitis and arthritis.<ref>PMID:10642520</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Bone sialoprotein-binding protein (Bbp), a MSCRAMMs (Microbial Surface Components Recognizing Adhesive Matrix Molecules) family protein expressed on the surface of Staphylococcus aureus (S. aureus), mediates adherence to fibrinogen alpha (Fg alpha), a component in the extracellular matrix of the host cell and is important for infection and pathogenesis. In this study, we solved the crystal structures of apo-Bbp(273-598) and Bbp(273-598)-Fg alpha(561-575) complex at a resolution of 2.03 A and 1.45 A, respectively. Apo-Bbp(273-598) contained the ligand binding region N2 and N3 domains, both of which followed a DE variant IgG fold characterized by an additional D1 strand in N2 domain and D1' and D2' strands in N3 domain. The peptide mapped to the Fg alpha(561-575) bond to Bbp(273-598) on the open groove between the N2 and N3 domains. Strikingly, the disordered C-terminus in the apo-form reorganized into a highly-ordered loop and a beta-strand G'' covering the ligand upon ligand binding. Bbp(Ala298-Gly301) in the N2 domain of the Bbp(273-598)-Fg alpha(561-575) complex, which is a loop in the apo-form, formed a short alpha-helix to interact tightly with the peptide. In addition, Bbp(Ser547-Gln561) in the N3 domain moved toward the binding groove to make contact directly with the peptide, while Bbp(Asp338-Gly355) and Bbp(Thr365-Tyr387) in N2 domain shifted their configurations to stabilize the reorganized C-terminus mainly through strong hydrogen bonds. Altogether, our results revealed the molecular basis for Bbp-ligand interaction and advanced our understanding of S. aureus infection process.
-Authors: Yu, Y., Zhang, X.Y., Gu, J.K.
+Crystal structures of Bbp from Staphylococcus aureus reveal the ligand binding mechanism with Fibrinogen alpha.,Zhang X, Wu M, Zhuo W, Gu J, Zhang S, Ge J, Yang M Protein Cell. 2015 Oct;6(10):757-66. doi: 10.1007/s13238-015-0205-x. Epub 2015, Sep 8. PMID:26349459<ref>PMID:26349459</ref>
-Description: Crystal structures of Bbp from Staphylococcus aureus
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Gu, J.K]]
+<div class="pdbe-citations 5cf3" style="background-color:#fffaf0;"></div>
-[[Category: Zhang, X.Y]]
+== References ==
-[[Category: Yu, Y]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Staphylococcus aureus]]
+[[Category: Gu JK]]
+[[Category: Yu Y]]
+[[Category: Zhang XY]]