5bpg: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 5bpg is ON HOLD
+==Crystal structure of the water-soluble FraC purified starting from the trans-membrane pore==
+<StructureSection load='5bpg' size='340' side='right'caption='[[5bpg]], [[Resolution|resolution]] 2.14&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5bpg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinia_fragacea Actinia fragacea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BPG FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.14&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bpg OCA], [https://pdbe.org/5bpg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bpg RCSB], [https://www.ebi.ac.uk/pdbsum/5bpg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bpg ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ACTPC_ACTFR ACTPC_ACTFR] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of several monomers.<ref>PMID:19563820</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The bidirectional transformation of a protein between its native water-soluble and integral transmembrane conformations is demonstrated for FraC, a hemolytic protein of the family of pore-forming toxins. In the presence of biological membranes, the water-soluble conformation of FraC undergoes a remarkable structural reorganization generating cytolytic transmembrane nanopores conducive to cell death. So far, the reverse transformation from the native transmembrane conformation to the native water-soluble conformation has not been reported. We describe the use of detergents with different physicochemical properties to achieve the spontaneous conversion of transmembrane pores of FraC back into the initial water-soluble state. Thermodynamic and kinetic stability data suggest that specific detergents cause an asymmetric change in the energy landscape of the protein, allowing the bidirectional transformation of a membrane protein.
-Authors: Caaveiro, J.M.M., Tanaka, K., Tsumoto, K.
+Bidirectional Transformation of a Metamorphic Protein between the Water-Soluble and Transmembrane Native States.,Tanaka K, Caaveiro JM, Tsumoto K Biochemistry. 2015 Nov 24;54(46):6863-6. doi: 10.1021/acs.biochem.5b01112. Epub, 2015 Nov 11. PMID:26544760<ref>PMID:26544760</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Caaveiro, J.M.M]]
+<div class="pdbe-citations 5bpg" style="background-color:#fffaf0;"></div>
-[[Category: Tanaka, K]]
-[[Category: Tsumoto, K]]
+==See Also==
+*[[Cytolysin 3D structures|Cytolysin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Actinia fragacea]]
+[[Category: Large Structures]]
+[[Category: Caaveiro JMM]]
+[[Category: Tanaka K]]
+[[Category: Tsumoto K]]