5boe: Difference between revisions

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-'''Unreleased structure'''
-The entry 5boe is ON HOLD
+==Crystal structure of Staphylococcus aureus enolase in complex with PEP==
+<StructureSection load='5boe' size='340' side='right'caption='[[5boe]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5boe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BOE FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5boe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5boe OCA], [https://pdbe.org/5boe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5boe RCSB], [https://www.ebi.ac.uk/pdbsum/5boe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5boe ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ENO_STAAU ENO_STAAU] Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis (By similarity). Binds laminin when expressed on the bacterial cell surface; this probably induces destruction of the extracellular matrix, favoring invasion and dissemination.[HAMAP-Rule:MF_00318]<ref>PMID:15158195</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Staphylococcus aureus is a Gram-positive bacterium with strong pathogenicity that causes a wide range of infections and diseases. Enolase is an evolutionarily conserved enzyme that plays a key role in energy production through glycolysis. Additionally, enolase is located on the surface of S. aureus and is involved in processes leading to infection. Here, crystal structures of Sa_enolase with and without bound phosphoenolpyruvate (PEP) are presented at 1.6 and 2.45 A resolution, respectively. The structure reveals an octameric arrangement; however, both dimeric and octameric conformations were observed in solution. Furthermore, enzyme-activity assays show that only the octameric variant is catalytically active. Biochemical and structural studies indicate that the octameric form of Sa_enolase is enzymatically active in vitro and likely also in vivo, while the dimeric form is catalytically inactive and may be involved in other biological processes.
-Authors: Wang, C.L., Wu, Y.F., Han, L., Wu, M.H., Zhang, X., Zang, J.Y.
+Octameric structure of Staphylococcus aureus enolase in complex with phosphoenolpyruvate.,Wu Y, Wang C, Lin S, Wu M, Han L, Tian C, Zhang X, Zang J Acta Crystallogr D Biol Crystallogr. 2015 Dec 1;71(Pt 12):2457-70. doi:, 10.1107/S1399004715018830. Epub 2015 Nov 26. PMID:26627653<ref>PMID:26627653</ref>
-Description: Crystal structure of Staphylococcus aureus enolase in complex with PEP
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Wang, C.L]]
+<div class="pdbe-citations 5boe" style="background-color:#fffaf0;"></div>
-[[Category: Zhang, X]]
-[[Category: Zang, J.Y]]
+==See Also==
-[[Category: Wu, Y.F]]
+*[[Enolase 3D structures|Enolase 3D structures]]
-[[Category: Wu, M.H]]
+== References ==
-[[Category: Han, L]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Staphylococcus aureus]]
+[[Category: Han L]]
+[[Category: Wang CL]]
+[[Category: Wu MH]]
+[[Category: Wu YF]]
+[[Category: Zang JY]]
+[[Category: Zhang X]]