5b18: Difference between revisions

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-'''Unreleased structure'''
-The entry 5b18 is ON HOLD
+==Crystal Structure of a Darunavir Resistant HIV-1 Protease==
+<StructureSection load='5b18' size='340' side='right'caption='[[5b18]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5b18]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B18 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b18 OCA], [https://pdbe.org/5b18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b18 RCSB], [https://www.ebi.ac.uk/pdbsum/5b18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b18 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/E5RVX9_9HIV1 E5RVX9_9HIV1]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Darunavir (DRV) is one of the most powerful protease inhibitors (PIs) for treating human immunodeficiency virus type-1 (HIV-1) infection and presents a high genetic barrier to the generation of resistant viruses. However, DRV-resistant HIV-1 infrequently emerges from viruses exhibiting resistance to other protease inhibitors. To address this resistance, researchers have gathered genetic information on DRV resistance. In contrast, few structural insights into the mechanism underlying DRV resistance are available. To elucidate this mechanism, we determined the crystal structure of the ligand-free state of a protease with high-level DRV resistance and six DRV resistance-associated mutations (including I47V and I50V), which we generated by in vitro selection. This crystal structure showed a unique curling conformation at the flap regions that was not found in the previously reported ligand-free protease structures. Molecular dynamics simulations indicated that the curled flap conformation altered the flap dynamics. These results suggest that the preference for a unique flap conformation influences DRV binding. These results provide new structural insights into elucidating the molecular mechanism of DRV resistance and aid to develop PIs effective against DRV-resistant viruses.
-Authors: Suzuki, K., Ode, H., Suzuki, A., Watanabe, N., Sugiura, W.
+Unique Flap Conformation in an HIV-1 Protease with High-Level Darunavir Resistance.,Nakashima M, Ode H, Suzuki K, Fujino M, Maejima M, Kimura Y, Masaoka T, Hattori J, Matsuda M, Hachiya A, Yokomaku Y, Suzuki A, Watanabe N, Sugiura W, Iwatani Y Front Microbiol. 2016 Feb 3;7:61. doi: 10.3389/fmicb.2016.00061. eCollection, 2016. PMID:26870021<ref>PMID:26870021</ref>
-Description: Crystal Structure of a Darunavir Resistant HIV-1 Protease
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Watanabe, N]]
+<div class="pdbe-citations 5b18" style="background-color:#fffaf0;"></div>
-[[Category: Ode, H]]
-[[Category: Suzuki, K]]
+==See Also==
-[[Category: Sugiura, W]]
+*[[Immunodeficiency virus protease 3D structures|Immunodeficiency virus protease 3D structures]]
-[[Category: Suzuki, A]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Human immunodeficiency virus 1]]
+[[Category: Large Structures]]
+[[Category: Iwatani Y]]
+[[Category: Nakashima M]]
+[[Category: Ode H]]
+[[Category: Sugiura W]]
+[[Category: Suzuki A]]
+[[Category: Suzuki K]]
+[[Category: Watanabe N]]