5b0l: Difference between revisions
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New page: '''Unreleased structure''' The entry 5b0l is ON HOLD until Paper Publication Authors: Ko, T.-P., Zhang, L., Chen, C.-C., Guo, R.-T., Oldfield, E.O. Description: [[Category: Unreleased... |
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==Structure of MoeN5-Sso7d fusion protein in complex with beta-nonyl glucoside== | |||
<StructureSection load='5b0l' size='340' side='right'caption='[[5b0l]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5b0l]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2] and [https://en.wikipedia.org/wiki/Streptomyces_viridosporus Streptomyces viridosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B0L FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b0l OCA], [https://pdbe.org/5b0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b0l RCSB], [https://www.ebi.ac.uk/pdbsum/5b0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b0l ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A010_STRVD A0A010_STRVD] [https://www.uniprot.org/uniprot/DN7D_SACS2 DN7D_SACS2] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature (By similarity). Stimulates the Holliday junction cleavage activity of Hjc (PubMed:11709558).[UniProtKB:P61990]<ref>PMID:11709558</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structure of MoeN5, a unique prenyltransferase involved in the biosynthesis of the antibiotic moenomycin, is reported. MoeN5 catalyzes the reaction of geranyl diphosphate (GPP) with the cis-farnesyl group in phosphoglycolipid 5 to form the (C25 ) moenocinyl-sidechain-containing lipid 7. GPP binds to an allylic site (S1) and aligns well with known S1 inhibitors. Alkyl glycosides, glycolipids, can bind to both S1 and a second site, S2. Long sidechains in S2 are "bent" and co-locate with the homoallylic substrate isopentenyl diphosphate in other prenyltransferases. These observations support a MoeN5 mechanism in which 5 binds to S2 with its C6-C11 group poised to attack C1 in GPP to form the moenocinyl sidechain, with the more distal regions of 5 aligning with the distal glucose in decyl maltoside. The results are of general interest because they provide the first structures of MoeN5 and a structural basis for its mechanism of action, results that will facilitate the design of new antibiotics. | |||
Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5.,Zhang L, Chen CC, Ko TP, Huang JW, Zheng Y, Liu W, Wang I, Malwal SR, Feng X, Wang K, Huang CH, Hsu ST, Wang AH, Oldfield E, Guo RT Angew Chem Int Ed Engl. 2016 Apr 4;55(15):4716-20. doi: 10.1002/anie.201511388., Epub 2016 Mar 8. PMID:26954060<ref>PMID:26954060</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Chen | <div class="pdbe-citations 5b0l" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Ko | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Saccharolobus solfataricus P2]] | |||
[[Category: Streptomyces viridosporus]] | |||
[[Category: Chen C-C]] | |||
[[Category: Guo R-T]] | |||
[[Category: Ko T-P]] | |||
[[Category: Oldfield EO]] | |||
[[Category: Zhang L]] | |||