4xpr: Difference between revisions
New page: '''Unreleased structure''' The entry 4xpr is ON HOLD Authors: Miyazaki, T., Ishizaki, Y., Ichikawa, M., Nishikawa, A., Tonozuka, T. Description: Category: Unreleased Structures [[... |
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The | ==Crystal structure of the mutant D365A of Pedobacter saltans GH31 alpha-galactosidase== | ||
<StructureSection load='4xpr' size='340' side='right'caption='[[4xpr]], [[Resolution|resolution]] 2.01Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4xpr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudopedobacter_saltans Pseudopedobacter saltans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XPR FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xpr OCA], [https://pdbe.org/4xpr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xpr RCSB], [https://www.ebi.ac.uk/pdbsum/4xpr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xpr ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0F7R6D6_9SPHI A0A0F7R6D6_9SPHI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Glycoside hydrolase family 31 (GH31) proteins have been reportedly identified as exo-alpha-glycosidases with activity for alpha-glucosides and alpha-xylosides. We focused on a GH31 subfamily, which contains proteins with low sequence identity (<24%) to the previously reported GH31 glycosidases, and characterized two enzymes from Pedobacter heparinus and Pedobacter saltans, respectively. The enzymes unexpectedly exhibited alpha-galactosidase activity, but were not active on alpha-glucosides and alpha-xylosides. The crystal structures of one of the enzymes, PsGal31A, in unliganded form and in complexes with D-galactose or L-fucose, and the catalytic nucleophile mutant in unliganded form and in complex with p-nitrophenyl-alpha-D-galactopyranoside, were determined at 1.85-2.30 A resolution. The overall structure of PsGal31A contains four domains, and the catalytic domain adopts a (beta/alpha)8-barrel fold that resembles the structures of other GH31 enzymes. Two catalytic aspartic acid residues are structurally conserved in the enzymes, whereas most residues forming the active site differ from those of GH31 alpha-glucosidases and alpha-xylosidases. PsGal31A forms a dimer via a unique loop that is not conserved in other reported GH31 enzymes; this loop is involved in its aglycone specificity and in binding L-fucose. Considering potential genes for alpha-L-fucosidases and carbohydrate-related proteins within the vicinity of Pedobacter Gal31, the identified Gal31 enzymes are likely to function in a novel sugar degradation system. This is the first report of alpha-galactosidases which belong to GH31 family. | |||
Structural and biochemical characterization of novel bacterial alpha-galactosidases belonging to glycoside hydrolase family 31.,Miyazaki T, Ishizaki Y, Ichikawa M, Nishikawa A, Tonozuka T Biochem J. 2015 May 5. PMID:25942325<ref>PMID:25942325</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 4xpr" style="background-color:#fffaf0;"></div> | ||
[[Category: Ichikawa | |||
[[Category: | ==See Also== | ||
[[Category: Miyazaki | *[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]] | ||
[[Category: | *[[Galactosidase 3D structures|Galactosidase 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudopedobacter saltans]] | |||
[[Category: Ichikawa M]] | |||
[[Category: Ishizaki Y]] | |||
[[Category: Miyazaki T]] | |||
[[Category: Nishikawa A]] | |||
[[Category: Tonozuka T]] | |||