4raf: Difference between revisions
New page: '''Unreleased structure''' The entry 4raf is ON HOLD Authors: Pan, D.H., Tang, J.Y., Xu, Y.F., Xiao, P., Liu, H.D., Wang, H.A., Wang, W.B., Meng, F.G., Yu, X., Sun, J.P. Description: C... |
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==Crystal structure of PP2Ca-D38A== | |||
<StructureSection load='4raf' size='340' side='right'caption='[[4raf]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4raf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RAF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RAF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.001Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4raf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4raf OCA], [https://pdbe.org/4raf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4raf RCSB], [https://www.ebi.ac.uk/pdbsum/4raf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4raf ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PPM1A_HUMAN PPM1A_HUMAN] Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling.<ref>PMID:16751101</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
PP2C family phosphatases (the type 2C family of protein phosphatases; or metal-dependent phosphatase, PPM) constitute an important class of signaling enzymes that regulate many fundamental life activities. All PP2C family members have a conserved binuclear metal ion active center that is essential for their catalysis. However, the catalytic role of each metal ion during catalysis remains elusive. In this study, we discovered that mutations in the structurally buried D38 residue of PP2Calpha (PPM1A) redefined the water-mediated hydrogen network in the active site and selectively disrupted M2 metal ion binding. Using the D38A and D38K mutations of PP2Calpha as specific tools in combination with enzymology analysis, our results demonstrated that the M2 metal ion determines the rate-limiting step of substrate hydrolysis, participates in dianion substrate binding and stabilizes the leaving group after P-O bond cleavage. The newly characterized catalytic role of the M2 metal ion in this family not only provides insight into how the binuclear metal centers of the PP2C phosphatases are organized for efficient catalysis but also helps increase our understanding of the function and substrate specificity of PP2C family members. | |||
The catalytic role of the M2 metal ion in PP2Calpha.,Pan C, Tang JY, Xu YF, Xiao P, Liu HD, Wang HA, Wang WB, Meng FG, Yu X, Sun JP Sci Rep. 2015 Feb 24;5:8560. doi: 10.1038/srep08560. PMID:25708299<ref>PMID:25708299</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4raf" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Liu HD]] | |||
[[Category: Meng FG]] | |||
[[Category: Pan C]] | |||
[[Category: Sun JP]] | |||
[[Category: Tang JY]] | |||
[[Category: Wang HA]] | |||
[[Category: Wang WB]] | |||
[[Category: Xiao P]] | |||
[[Category: Xu YF]] | |||
[[Category: Yu X]] | |||