4n8c: Difference between revisions
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==Three-dimensional structure of the extracellular domain of Matrix protein 2 of influenza A virus== | ==Three-dimensional structure of the extracellular domain of Matrix protein 2 of influenza A virus== | ||
<StructureSection load='4n8c' size='340' side='right' caption='[[4n8c]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='4n8c' size='340' side='right'caption='[[4n8c]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4n8c]] is a 6 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4n8c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Puerto_Rico/8/1934(H1N1)) Influenza A virus (A/Puerto Rico/8/1934(H1N1))] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N8C FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n8c OCA], [https://pdbe.org/4n8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n8c RCSB], [https://www.ebi.ac.uk/pdbsum/4n8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n8c ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The extracellular domain of influenza A virus matrix protein 2 (M2e) is conserved and is being evaluated as a quasiuniversal influenza A vaccine candidate. We describe the crystal structure at 1.6 A resolution of M2e in complex with the Fab fragment of an M2e-specific monoclonal antibody that protects against influenza A virus challenge. This antibody binds M2 expressed on the surfaces of cells infected with influenza A virus. Five out of six complementary determining regions interact with M2e, and three highly conserved M2e residues are critical for this interaction. In this complex, M2e adopts a compact U-shaped conformation stabilized in the center by the highly conserved tryptophan residue in M2e. This is the first description of the three-dimensional structure of M2e. IMPORTANCE: M2e of influenza A is under investigation as a universal influenza A vaccine, but its three-dimensional structure is unknown. We describe the structure of M2e stabilized with an M2e-specific monoclonal antibody that recognizes natural M2. We found that the conserved tryptophan is positioned in the center of the U-shaped structure of M2e and stabilizes its conformation. The structure also explains why previously reported in vivo escape viruses, selected with a similar monoclonal antibody, carried proline residue substitutions at position 10 in M2. | |||
Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody.,Cho KJ, Schepens B, Seok JH, Kim S, Roose K, Lee JH, Gallardo R, Van Hamme E, Schymkowitz J, Rousseau F, Fiers W, Saelens X, Kim KH J Virol. 2015 Apr;89(7):3700-11. doi: 10.1128/JVI.02576-14. Epub 2015 Jan 21. PMID:25609808<ref>PMID:25609808</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4n8c" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Cho | [[Category: Cho KJ]] | ||
[[Category: Fiers | [[Category: Fiers W]] | ||
[[Category: Kim | [[Category: Kim KH]] | ||
[[Category: Kim | [[Category: Kim S]] | ||
[[Category: Roose | [[Category: Roose K]] | ||
[[Category: Saelens | [[Category: Saelens X]] | ||
[[Category: Schepens | [[Category: Schepens B]] | ||
[[Category: Seok | [[Category: Seok JH]] | ||
Latest revision as of 17:46, 8 November 2023
Three-dimensional structure of the extracellular domain of Matrix protein 2 of influenza A virusThree-dimensional structure of the extracellular domain of Matrix protein 2 of influenza A virus
Structural highlights
Publication Abstract from PubMedThe extracellular domain of influenza A virus matrix protein 2 (M2e) is conserved and is being evaluated as a quasiuniversal influenza A vaccine candidate. We describe the crystal structure at 1.6 A resolution of M2e in complex with the Fab fragment of an M2e-specific monoclonal antibody that protects against influenza A virus challenge. This antibody binds M2 expressed on the surfaces of cells infected with influenza A virus. Five out of six complementary determining regions interact with M2e, and three highly conserved M2e residues are critical for this interaction. In this complex, M2e adopts a compact U-shaped conformation stabilized in the center by the highly conserved tryptophan residue in M2e. This is the first description of the three-dimensional structure of M2e. IMPORTANCE: M2e of influenza A is under investigation as a universal influenza A vaccine, but its three-dimensional structure is unknown. We describe the structure of M2e stabilized with an M2e-specific monoclonal antibody that recognizes natural M2. We found that the conserved tryptophan is positioned in the center of the U-shaped structure of M2e and stabilizes its conformation. The structure also explains why previously reported in vivo escape viruses, selected with a similar monoclonal antibody, carried proline residue substitutions at position 10 in M2. Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody.,Cho KJ, Schepens B, Seok JH, Kim S, Roose K, Lee JH, Gallardo R, Van Hamme E, Schymkowitz J, Rousseau F, Fiers W, Saelens X, Kim KH J Virol. 2015 Apr;89(7):3700-11. doi: 10.1128/JVI.02576-14. Epub 2015 Jan 21. PMID:25609808[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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