4k1v: Difference between revisions
New page: '''Unreleased structure''' The entry 4k1v is ON HOLD Authors: Cha, H.J., Jang, D.S, Kim, Y.-G., Hong, B.H., Woo, J.-S., Choi, K.Y. Description: Crystal structure of delta5-3-ketosteroi... |
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==Crystal structure of delta5-3-ketosteroid isomerase containing Y16F and Y57F mutations== | |||
<StructureSection load='4k1v' size='340' side='right'caption='[[4k1v]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4k1v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K1V FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1v OCA], [https://pdbe.org/4k1v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k1v RCSB], [https://www.ebi.ac.uk/pdbsum/4k1v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k1v ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/SDIS_PSEPU SDIS_PSEPU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Proteins have evolved to compensate for detrimental mutations. However, compensatory mechanisms for protein defects are not well understood. Using ketosteroid isomerase (KSI), we investigated how second-site mutations could recover defective mutant function and stability. Previous results revealed that the Y30F mutation rescued the Y14F, Y55F and Y14F/Y55F mutants by increasing the catalytic activity by 23-, 3- and 1.3-fold, respectively, and the Y55F mutant by increasing the stability by 3.3 kcal/mol. To better understand these observations, we systematically investigated detailed structural and thermodynamic effects of the Y30F mutation on these mutants. Crystal structures of the Y14F/Y30F and Y14F/Y55F mutants were solved at 2.0 and 1.8 previoulsy solved structures of wild-type and other mutant KSIs. Structural analyses revealed that the Y30F mutation partially restored the active-site cleft of these mutant KSIs. The Y30F mutation also increased Y14F and Y14F/Y55F mutant stability by 3.2 and 4.3 kcal/mol, respectively, and the melting temperatures of the Y14F, Y55F and Y14F/Y55F mutants by 6.4 degrees C, 5.1 degrees C and 10.0 degrees C, respectively. Compensatory effects of the Y30F mutation on stability might be due to improved hydrophobic interactions because removal of a hydroxyl group from Tyr30 induced local compaction by neighboring residue movement and enhanced interactions with surrounding hydrophobic residues in the active site. Taken together, our results suggest that perturbed active-site geometry recovery and favorable hydrophobic interactions mediate the role of Y30F as a secondsite suppressor. | |||
Rescue of deleterious mutations by the compensatory Y30F mutation in ketosteroid isomerase.,Cha HJ, Jang do S, Kim YG, Hong BH, Woo JS, Kim KT, Choi KY Mol Cells. 2013 Jul;36(1):39-46. doi: 10.1007/s10059-013-0013-1. Epub 2013 Jun 3. PMID:23740430<ref>PMID:23740430</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4k1v" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ketosteroid Isomerase|Ketosteroid Isomerase]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas putida]] | |||
[[Category: Cha HJ]] | |||
[[Category: Choi KY]] | |||
[[Category: Hong BH]] | |||
[[Category: Jang DS]] | |||
[[Category: Kim YG]] | |||
[[Category: Woo JS]] | |||