4e19: Difference between revisions

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==Crystal structure of RNase H1 from halophilic archaeon Halobacterium salinarum NRC-1==
==Crystal structure of RNase H1 from halophilic archaeon Halobacterium salinarum NRC-1==
<StructureSection load='4e19' size='340' side='right' caption='[[4e19]], [[Resolution|resolution]] 1.41&Aring;' scene=''>
<StructureSection load='4e19' size='340' side='right'caption='[[4e19]], [[Resolution|resolution]] 1.41&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4e19]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Halobacterium_sp._nrc-1 Halobacterium sp. nrc-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E19 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4e19]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum_NRC-1 Halobacterium salinarum NRC-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E19 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.41&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VNG_0255C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64091 Halobacterium sp. NRC-1])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e19 OCA], [https://pdbe.org/4e19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e19 RCSB], [https://www.ebi.ac.uk/pdbsum/4e19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e19 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e19 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e19 RCSB], [http://www.ebi.ac.uk/pdbsum/4e19 PDBsum]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/RNH_HALSA RNH_HALSA]] Nuclease that specifically degrades the RNA of RNA-DNA hybrids; seems to act exonucleolytically on RNA/DNA hybrids. Endonucleolytically removes RNA primers from the Okazaki fragments of lagging strand synthesis on its own. Complements the temperature-sensitive phenotype of an E.coli double rnhA/rnhB (RNase H) disruption mutant.<ref>PMID:15115438</ref>
[https://www.uniprot.org/uniprot/RNH_HALSA RNH_HALSA] Nuclease that specifically degrades the RNA of RNA-DNA hybrids; seems to act exonucleolytically on RNA/DNA hybrids. Endonucleolytically removes RNA primers from the Okazaki fragments of lagging strand synthesis on its own. Complements the temperature-sensitive phenotype of an E.coli double rnhA/rnhB (RNase H) disruption mutant.<ref>PMID:15115438</ref>  


==See Also==
==See Also==
*[[Ribonuclease|Ribonuclease]]
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
*[[Temp|Temp]]
*[[User:Jaime.Prilusky/Test/tree|User:Jaime.Prilusky/Test/tree]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Halobacterium sp. nrc-1]]
[[Category: Halobacterium salinarum NRC-1]]
[[Category: Ribonuclease H]]
[[Category: Large Structures]]
[[Category: Angkawidjaja, C]]
[[Category: Angkawidjaja C]]
[[Category: Kanaya, S]]
[[Category: Kanaya S]]
[[Category: Koga, Y]]
[[Category: Koga Y]]
[[Category: You, D J]]
[[Category: You DJ]]
[[Category: Hydrolase]]
[[Category: Rnase h1]]

Latest revision as of 16:45, 8 November 2023

Crystal structure of RNase H1 from halophilic archaeon Halobacterium salinarum NRC-1Crystal structure of RNase H1 from halophilic archaeon Halobacterium salinarum NRC-1

Structural highlights

4e19 is a 2 chain structure with sequence from Halobacterium salinarum NRC-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.41Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RNH_HALSA Nuclease that specifically degrades the RNA of RNA-DNA hybrids; seems to act exonucleolytically on RNA/DNA hybrids. Endonucleolytically removes RNA primers from the Okazaki fragments of lagging strand synthesis on its own. Complements the temperature-sensitive phenotype of an E.coli double rnhA/rnhB (RNase H) disruption mutant.[1]

See Also

References

  1. ↑ Ohtani N, Yanagawa H, Tomita M, Itaya M. Identification of the first archaeal Type 1 RNase H gene from Halobacterium sp. NRC-1: archaeal RNase HI can cleave an RNA-DNA junction. Biochem J. 2004 Aug 1;381(Pt 3):795-802. PMID:15115438 doi:http://dx.doi.org/10.1042/BJ20040153

4e19, resolution 1.41Å

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