3wmq: Difference between revisions
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==Crystal structure of the complex between SLL-2 and GalNAc.== | |||
<StructureSection load='3wmq' size='340' side='right'caption='[[3wmq]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3wmq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinularia_lochmodes Sinularia lochmodes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WMQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WMQ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wmq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wmq OCA], [https://pdbe.org/3wmq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wmq RCSB], [https://www.ebi.ac.uk/pdbsum/3wmq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wmq ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A4CYJ6_9CNID A4CYJ6_9CNID] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
D-Galactose-binding lectin from the octocoral, Sinularia lochmodes (SLL-2), distributes densely on the cell surface of microalgae, Symbiodinium sp., an endosymbiotic dinoflagellate of the coral, and is also shown to be a chemical cue that transforms dinoflagellate into a non-motile (coccoid) symbiotic state. SLL-2 binds with high affinity to the Forssman antigen (N-acetylgalactosamine(GalNAc)alpha1-3GalNAcbeta1-3Galalpha1-4Galbeta1-4Glc-ceram ide), and the presence of Forssman antigen-like sugar on the surface of Symbiodinium CS-156 cells was previously confirmed. Here we report the crystal structures of SLL-2 and its GalNAc complex as the first crystal structures of a lectin involved in the symbiosis between coral and dinoflagellate. N-Linked sugar chains and a galactose derivative binding site common to H-type lectins were observed in each monomer of the hexameric SLL-2 crystal structure. In addition, unique sugar-binding site-like regions were identified at the top and bottom of the hexameric SLL-2 structure. These structural features suggest a possible binding mode between SLL-2 and Forssman antigen-like pentasaccharide. | |||
Crystal structure of a symbiosis-related lectin from octocoral.,Kita A, Jimbo M, Sakai R, Morimoto Y, Miki K Glycobiology. 2015 Sep;25(9):1016-23. doi: 10.1093/glycob/cwv033. Epub 2015 May, 28. PMID:26022515<ref>PMID:26022515</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3wmq" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Galactose-binding lectin|Galactose-binding lectin]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Sinularia lochmodes]] | |||
[[Category: Jimbo M]] | |||
[[Category: Kita A]] | |||
[[Category: Miki K]] | |||
[[Category: Morimoto Y]] | |||
[[Category: Sakai R]] | |||
Latest revision as of 16:15, 8 November 2023
Crystal structure of the complex between SLL-2 and GalNAc.Crystal structure of the complex between SLL-2 and GalNAc.
Structural highlights
FunctionPublication Abstract from PubMedD-Galactose-binding lectin from the octocoral, Sinularia lochmodes (SLL-2), distributes densely on the cell surface of microalgae, Symbiodinium sp., an endosymbiotic dinoflagellate of the coral, and is also shown to be a chemical cue that transforms dinoflagellate into a non-motile (coccoid) symbiotic state. SLL-2 binds with high affinity to the Forssman antigen (N-acetylgalactosamine(GalNAc)alpha1-3GalNAcbeta1-3Galalpha1-4Galbeta1-4Glc-ceram ide), and the presence of Forssman antigen-like sugar on the surface of Symbiodinium CS-156 cells was previously confirmed. Here we report the crystal structures of SLL-2 and its GalNAc complex as the first crystal structures of a lectin involved in the symbiosis between coral and dinoflagellate. N-Linked sugar chains and a galactose derivative binding site common to H-type lectins were observed in each monomer of the hexameric SLL-2 crystal structure. In addition, unique sugar-binding site-like regions were identified at the top and bottom of the hexameric SLL-2 structure. These structural features suggest a possible binding mode between SLL-2 and Forssman antigen-like pentasaccharide. Crystal structure of a symbiosis-related lectin from octocoral.,Kita A, Jimbo M, Sakai R, Morimoto Y, Miki K Glycobiology. 2015 Sep;25(9):1016-23. doi: 10.1093/glycob/cwv033. Epub 2015 May, 28. PMID:26022515[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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