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==Crystal structure of Agrocybe cylindracea galectin mutant (N46A) with blood type A antigen tetraose== | |||
<StructureSection load='3wg4' size='340' side='right'caption='[[3wg4]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3wg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrocybe_cylindracea Agrocybe cylindracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WG4 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRD_900132:Blood+group+A+H+type+2+antigen,+beta+anomer'>PRD_900132</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wg4 OCA], [https://pdbe.org/3wg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wg4 RCSB], [https://www.ebi.ac.uk/pdbsum/3wg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wg4 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ATLE_CYCAE ATLE_CYCAE] Anti-tumor lectin with DNase activity. Inhibits the growth of several tumor cell lines in vitro. Induces lymphocyte infiltration and necrosis of tumor cells in a mouse tumor model. Induces apoptosis in HeLa cells. Binds N-acetylneuraminyl lactose (N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucose) (PubMed:16051274).<ref>PMID:12757412</ref> <ref>PMID:16051274</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A fungal galectin from Agrocybe cylindracea (ACG) exhibits broad binding specificity for beta-galactose-containing glycans. We determined the crystal structures of wild-type ACG and the N46A mutant, with and without glycan ligands. From these structures and a saccharide-binding analysis of the N46A mutant, we revealed that a conformational change of a unique insertion sequence containing Asn46 provides two binding modes for ACG, and thereby confers broad binding specificity. We propose that the unique sequence provides these two distinct glycan-binding modes by an induced-fit mechanism. | |||
Conformational change of a unique sequence in a fungal galectin from Agrocybe cylindracea controls glycan ligand-binding specificity.,Kuwabara N, Hu D, Tateno H, Makyio H, Hirabayashi J, Kato R FEBS Lett. 2013 Nov 15;587(22):3620-5. doi: 10.1016/j.febslet.2013.08.046. Epub, 2013 Sep 10. PMID:24036446<ref>PMID:24036446</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3wg4" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Galectin 3D structures|Galectin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Agrocybe cylindracea]] | |||
[[Category: Large Structures]] | |||
[[Category: Hirabayashi J]] | |||
[[Category: Hu D]] | |||
[[Category: Kato R]] | |||
[[Category: Kuwabara N]] | |||
[[Category: Makio H]] | |||
[[Category: Tateno H]] | |||
Latest revision as of 16:08, 8 November 2023
Crystal structure of Agrocybe cylindracea galectin mutant (N46A) with blood type A antigen tetraoseCrystal structure of Agrocybe cylindracea galectin mutant (N46A) with blood type A antigen tetraose
Structural highlights
FunctionATLE_CYCAE Anti-tumor lectin with DNase activity. Inhibits the growth of several tumor cell lines in vitro. Induces lymphocyte infiltration and necrosis of tumor cells in a mouse tumor model. Induces apoptosis in HeLa cells. Binds N-acetylneuraminyl lactose (N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucose) (PubMed:16051274).[1] [2] Publication Abstract from PubMedA fungal galectin from Agrocybe cylindracea (ACG) exhibits broad binding specificity for beta-galactose-containing glycans. We determined the crystal structures of wild-type ACG and the N46A mutant, with and without glycan ligands. From these structures and a saccharide-binding analysis of the N46A mutant, we revealed that a conformational change of a unique insertion sequence containing Asn46 provides two binding modes for ACG, and thereby confers broad binding specificity. We propose that the unique sequence provides these two distinct glycan-binding modes by an induced-fit mechanism. Conformational change of a unique sequence in a fungal galectin from Agrocybe cylindracea controls glycan ligand-binding specificity.,Kuwabara N, Hu D, Tateno H, Makyio H, Hirabayashi J, Kato R FEBS Lett. 2013 Nov 15;587(22):3620-5. doi: 10.1016/j.febslet.2013.08.046. Epub, 2013 Sep 10. PMID:24036446[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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