3wbj: Difference between revisions
New page: '''Unreleased structure''' The entry 3wbj is ON HOLD Authors: Zheng, A., Yamamoto, R., Ose, T., Yu, J., Tanaka, I., Yao, M. Description: Crystal structure analysis of eukaryotic transl... |
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The | ==Crystal structure analysis of eukaryotic translation initiation factor 5B structure II== | ||
<StructureSection load='3wbj' size='340' side='right'caption='[[3wbj]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3wbj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WBJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WBJ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.495Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wbj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wbj OCA], [https://pdbe.org/3wbj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wbj RCSB], [https://www.ebi.ac.uk/pdbsum/3wbj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wbj ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/IF2P_YEAST IF2P_YEAST] Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
eIF5B and eIF1A are two translation-initiation factors that are universally conserved among all kingdoms. They show a unique interaction in eukaryotes which is important for ribosomal subunit joining. Here, the structures of two isolated forms of yeast eIF5B and of the eIF5B-eIF1A complex (eIF1A and eIF5B do not contain the respective N-terminal domains) are reported. The eIF5B-eIF1A structure shows that the C-terminal tail of eIF1A binds to eIF5B domain IV, while the core domain of eIF1A is invisible in the electron-density map. Although the individual domains in all structures of eIF5B or archaeal IF5B (aIF5B) are similar, their domain arrangements are significantly different, indicating high structural flexibility, which is advantageous for conformational change during ribosomal subunit joining. Based on these structures, models of eIF5B, eIF1A and tRNAi(Met) on the 80S ribosome were built. The models suggest that the interaction between the eIF1A C-terminal tail and eIF5B helps tRNAi(Met) to bind to eIF5B domain IV, thus preventing tRNAi(Met) dissociation, stabilizing the interface for subunit joining and providing a checkpoint for correct ribosome assembly. | |||
X-ray structures of eIF5B and the eIF5B-eIF1A complex: the conformational flexibility of eIF5B is restricted on the ribosome by interaction with eIF1A.,Zheng A, Yu J, Yamamoto R, Ose T, Tanaka I, Yao M Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3090-8. doi:, 10.1107/S1399004714021476. Epub 2014 Nov 22. PMID:25478828<ref>PMID:25478828</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3wbj" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae S288C]] | |||
[[Category: Ose T]] | |||
[[Category: Tanaka I]] | |||
[[Category: Yamamoto R]] | |||
[[Category: Yao M]] | |||
[[Category: Yu J]] | |||
[[Category: Zheng A]] | |||
Latest revision as of 16:02, 8 November 2023
Crystal structure analysis of eukaryotic translation initiation factor 5B structure IICrystal structure analysis of eukaryotic translation initiation factor 5B structure II
Structural highlights
FunctionIF2P_YEAST Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. Publication Abstract from PubMedeIF5B and eIF1A are two translation-initiation factors that are universally conserved among all kingdoms. They show a unique interaction in eukaryotes which is important for ribosomal subunit joining. Here, the structures of two isolated forms of yeast eIF5B and of the eIF5B-eIF1A complex (eIF1A and eIF5B do not contain the respective N-terminal domains) are reported. The eIF5B-eIF1A structure shows that the C-terminal tail of eIF1A binds to eIF5B domain IV, while the core domain of eIF1A is invisible in the electron-density map. Although the individual domains in all structures of eIF5B or archaeal IF5B (aIF5B) are similar, their domain arrangements are significantly different, indicating high structural flexibility, which is advantageous for conformational change during ribosomal subunit joining. Based on these structures, models of eIF5B, eIF1A and tRNAi(Met) on the 80S ribosome were built. The models suggest that the interaction between the eIF1A C-terminal tail and eIF5B helps tRNAi(Met) to bind to eIF5B domain IV, thus preventing tRNAi(Met) dissociation, stabilizing the interface for subunit joining and providing a checkpoint for correct ribosome assembly. X-ray structures of eIF5B and the eIF5B-eIF1A complex: the conformational flexibility of eIF5B is restricted on the ribosome by interaction with eIF1A.,Zheng A, Yu J, Yamamoto R, Ose T, Tanaka I, Yao M Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3090-8. doi:, 10.1107/S1399004714021476. Epub 2014 Nov 22. PMID:25478828[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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