3vu0: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:3vu0.jpg|left|200px]]
-{{STRUCTURE_3vu0|  PDB=3vu0  |  SCENE=  }}
+==Crystal structure of the C-terminal globular domain of oligosaccharyltransferase (AfAglB-S2, AF_0040, O30195_ARCFU) from Archaeoglobus fulgidus==
+<StructureSection load='3vu0' size='340' side='right'caption='[[3vu0]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3vu0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VU0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu0 OCA], [https://pdbe.org/3vu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vu0 RCSB], [https://www.ebi.ac.uk/pdbsum/3vu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vu0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AGLB2_ARCFU AGLB2_ARCFU] Oligosaccharyl transferase (OST) that catalyzes the initial transfer of a defined glycan (a GalNAc-linked heptasaccharide composed of 4 Hex, 3 dHex and a sulfate for A.fulgidus AglB-S) from the lipid carrier dolichol-monophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation.<ref>PMID:27015803</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of an oligosaccharide to an asparagine residue in glycoproteins. It possesses a binding pocket that recognizes Ser and Thr residues at the +2 position in the N-glycosylation consensus, Asn-X-Ser/Thr. We determined the crystal structures of the C-terminal globular domains of the catalytic subunits of two archaeal OSTs. A comparison with previously determined structures identified a segment with remarkable conformational plasticity, induced by crystal contact effects. We characterized its dynamic properties in solution by (15)N NMR relaxation analyses. Intriguingly, the mobile region contains the +2 Ser/Thr-binding pocket. In agreement, the flexibility restriction forced by an engineered disulfide crosslink abolished the enzymatic activity, and its cleavage fully restored activity. These results suggest the necessity of multiple conformational states in the reaction. The dynamic nature of the Ser/Thr pocket could facilitate the efficient scanning of N-glycosylation sequons along nascent polypeptide chains.
-===Crystal structure of the C-terminal globular domain of oligosaccharyltransferase (AfAglB-S2, AF_0040, O30195_ARCFU) from Archaeoglobus fulgidus===
+Crystallographic and NMR evidence for flexibility in oligosaccharyltransferases and its catalytic significance.,Nyirenda J, Matsumoto S, Saitoh T, Maita N, Noda NN, Inagaki F, Kohda D Structure. 2013 Jan 8;21(1):32-41. doi: 10.1016/j.str.2012.10.011. Epub 2012 Nov , 21. PMID:23177926<ref>PMID:23177926</ref>
-{{ABSTRACT_PUBMED_23177926}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 3vu0" style="background-color:#fffaf0;"></div>
-[[3vu0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU0 OCA].
+== References ==
-[[Category: Archaeoglobus fulgidus dsm 4304]]
+<references/>
-[[Category: Dolichyl-diphosphooligosaccharide--protein glycotransferase]]
+__TOC__
-[[Category: Inagaki, F.]]
+</StructureSection>
-[[Category: Kohda, D.]]
+[[Category: Archaeoglobus fulgidus DSM 4304]]
-[[Category: Maita, N.]]
+[[Category: Large Structures]]
-[[Category: Matsumoto, S.]]
+[[Category: Inagaki F]]
-[[Category: Noda, N N.]]
+[[Category: Kohda D]]
-[[Category: Nyirenda, J.]]
+[[Category: Maita N]]
-[[Category: Saitoh, T.]]
+[[Category: Matsumoto S]]
-[[Category: Asn-glycosylation]]
+[[Category: Noda NN]]
-[[Category: Glycotransferase]]
+[[Category: Nyirenda J]]
-[[Category: Membrane]]
+[[Category: Saitoh T]]
-[[Category: Transferase]]