5mkp: Difference between revisions
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==Non redox thiolation in transfer RNAs occuring via sulfur activation by a [4Fe-4S] cluster== | ==Non redox thiolation in transfer RNAs occuring via sulfur activation by a [4Fe-4S] cluster== | ||
<StructureSection load='5mkp' size='340' side='right' caption='[[5mkp]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='5mkp' size='340' side='right'caption='[[5mkp]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5mkp]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5mkp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MKP FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=Q46:Fe4+H+S5'>Q46</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
< | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mkp OCA], [https://pdbe.org/5mkp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mkp RCSB], [https://www.ebi.ac.uk/pdbsum/5mkp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mkp ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/TTUA_PYRHO TTUA_PYRHO] Catalyzes the ATP-dependent 2-thiolation of 5-methyluridine residue at position 54 in the T loop of tRNAs, leading to 5-methyl-2-thiouridine (m(5)s(2)U or s(2)T) (PubMed:28655838). This modification allows thermal stabilization of tRNAs in thermophilic microorganisms, and is required for cell growth at high temperatures (By similarity). Can use free sulfide as sulfur source in vitro, which may be also the sulfur source in vivo (PubMed:28655838).[UniProtKB:Q72LF3]<ref>PMID:28655838</ref> | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Pyrococcus horikoshii]] | [[Category: Large Structures]] | ||
[[Category: Arragain | [[Category: Pyrococcus horikoshii OT3]] | ||
[[Category: Bimai | [[Category: Arragain S]] | ||
[[Category: Golinelli-Pimpaneau | [[Category: Bimai O]] | ||
[[Category: Legrand | [[Category: Golinelli-Pimpaneau B]] | ||
[[Category: Legrand P]] | |||