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==Crystal structure (Type-1) of SAICAR synthetase from Pyrococcus horikoshii OT3== | |||
<StructureSection load='3u54' size='340' side='right'caption='[[3u54]], [[Resolution|resolution]] 2.35Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3u54]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U54 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u54 OCA], [https://pdbe.org/3u54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u54 RCSB], [https://www.ebi.ac.uk/pdbsum/3u54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u54 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PUR7_PYRHO PUR7_PYRHO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The first native crystal structure of Phosphoribosylaminoimidazole-succinocarboxamide synthetase (SAICAR synthetase) from a hyperthermophilic organism Pyrococcus horikoshii OT3 was determined in two space groups H3 (Type-1: Resolution 2.35A) and in C222(1) (Type-2: Resolution 1.9A). Both are dimeric but Type-1 structure exhibited hexameric arrangement due to the presence of cadmium ions. A comparison has been made on the sequence and structures of all SAICAR synthetases to better understand the differences between mesophilic, thermophilic and hyperthermophilic SAICAR synthetases. These SAICAR synthetases are reasonably similar in sequence and three-dimensional structure; however, differences were visible only in the subtler details of percentage composition of the sequences, salt bridge interactions and non-polar contact areas. | |||
Structure of SAICAR synthetase from Pyrococcus horikoshii OT3: insights into thermal stability.,Manjunath K, Kanaujia SP, Kanagaraj S, Jeyakanthan J, Sekar K Int J Biol Macromol. 2013 Feb;53:7-19. doi: 10.1016/j.ijbiomac.2012.10.028. Epub , 2012 Nov 5. PMID:23137517<ref>PMID:23137517</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3u54" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[SAICAR synthetase|SAICAR synthetase]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pyrococcus horikoshii OT3]] | |||
[[Category: Jeyakanthan J]] | |||
[[Category: Kanagaraj S]] | |||
[[Category: Kanaujia SP]] | |||
[[Category: Manjunath K]] | |||
[[Category: Sekar K]] | |||
Latest revision as of 20:34, 1 November 2023
Crystal structure (Type-1) of SAICAR synthetase from Pyrococcus horikoshii OT3Crystal structure (Type-1) of SAICAR synthetase from Pyrococcus horikoshii OT3
Structural highlights
FunctionPublication Abstract from PubMedThe first native crystal structure of Phosphoribosylaminoimidazole-succinocarboxamide synthetase (SAICAR synthetase) from a hyperthermophilic organism Pyrococcus horikoshii OT3 was determined in two space groups H3 (Type-1: Resolution 2.35A) and in C222(1) (Type-2: Resolution 1.9A). Both are dimeric but Type-1 structure exhibited hexameric arrangement due to the presence of cadmium ions. A comparison has been made on the sequence and structures of all SAICAR synthetases to better understand the differences between mesophilic, thermophilic and hyperthermophilic SAICAR synthetases. These SAICAR synthetases are reasonably similar in sequence and three-dimensional structure; however, differences were visible only in the subtler details of percentage composition of the sequences, salt bridge interactions and non-polar contact areas. Structure of SAICAR synthetase from Pyrococcus horikoshii OT3: insights into thermal stability.,Manjunath K, Kanaujia SP, Kanagaraj S, Jeyakanthan J, Sekar K Int J Biol Macromol. 2013 Feb;53:7-19. doi: 10.1016/j.ijbiomac.2012.10.028. Epub , 2012 Nov 5. PMID:23137517[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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